Genetic polymorphisms in DNA repair genes and possible links with DNA repair rates, chromosomal aberrations and single-strand breaks in DNA
2003; Oxford University Press; Volume: 25; Issue: 5 Linguagem: Inglês
10.1093/carcin/bgh064
ISSN1460-2180
AutoresPavel Vodiĉka, Rajiv Kumar, Rudolf Štětina, Somali Sanyal, Pavel Souček, Vincent Haufroid, Mária Dušinská, Miroslava Kuricová, Maria Zamecnikova, Ľudovít Mušák, Jana Buchancova, Hannu Norppa, A. Hirvonen, Ľudmila Vodičková, Alessio Naccarati, Zora Matousu, Kari Hemminki,
Tópico(s)Acute Lymphoblastic Leukemia research
ResumoWe analysed the associations between genetic polymorphisms in genes coding for DNA repair enzymes XPD (exon 23 A → C, K751Q), XPG (exon 15 G → C, D1104H), XPC (exon 15 A → C, K939Q), XRCC1 (exon 10 G → A, R399Q) and XRCC3 (exon 7 C → T, T241 M) and the levels of chromosomal aberrations (CAs) and single-strand breaks (SSBs) in peripheral lymphocytes in a central European population. We also measured the irradiation-specific DNA repair rates and the repair rates of 8-oxoguanines in these individuals. An elevated frequency of CAs was observed in individuals with the XPD exon 23 A allele ( AA and AC ) genotypes ( F = 3.6, P = 0.028, ANOVA). In multifactorial analysis of variance, the XPD exon 23 polymorphism appeared as a major factor influencing CAs ( F = 4.2, P = 0.017). SSBs in DNA, on the other hand, were modulated by XPD ( F = 4.3, P = 0.023), XPG ( F = 4.3, P = 0.024) and XRCC1 genotypes ( F = 3.0, P = 0.064). Irradiation-specific DNA repair rates (reflecting mainly base excision repair activity) were affected by XRCC1 ( F = 5.9, P = 0.010) and XPC polymorphisms ( F = 4.2, P = 0.046, MANOVA). Our results from this study suggest that markers of genotoxicity are associated with polymorphisms in genes encoding DNA repair enzymes.
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