Rapid laboratory identification and characterization of verocytotoxigenic (Shiga toxin producing) Escherichia coli (VTEC/STEC)
2003; Oxford University Press; Volume: 95; Issue: 2 Linguagem: Inglês
10.1046/j.1365-2672.2003.02031.x
ISSN1365-2672
AutoresK. A. Bettelheim, Lothar Beutin,
Tópico(s)Bacterial Genetics and Biotechnology
Resumo1. Summary, 205 2. Introduction, 205 2.1 VTEC, a brief history, 206 2.2 The Vero (Shiga) toxin family, 206 2.3 VTEC O157: the confounding variable, 207 3. Current methods for identification of VTEC in samples of different origin, 207 3.1 DNA-based methodology for identification of VTEC by detection of VT/Stx genes, 208 3.2 Tissue culture methods to detect VTEC by their toxic effects on mammalian cells, 208 3.3 Immunological methods to detect Verotoxins with VT/Stx specific antibodies, 209 4. Immunological test kits, 209 4.1 Premier EHEC, 209 4.2 The Ridascreen Ò Verotoxin enzyme immunoassay technique, 209 4.3 The PROSPECT Ò Shiga toxin (STEC) microplate assay, 210 4.4 The VTEC-Screen 'SEIKEN', 210 4.5 An adaptation of the VTEC-Screen 'SEIKEN', 210 5. Combined methods for detection and isolation of VTEC/ STEC from test samples, 211 5.1 The Shiga toxin (Verotoxin) colony immunoblot, 211 6.Combined serological and cultural methods for the isolation of VTEC, 211 7. Consequences for routine clinical laboratories involved in VTEC diagnosis, 212 8. References,
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