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PTRF-Cavin, a Conserved Cytoplasmic Protein Required for Caveola Formation and Function

2008; Cell Press; Volume: 132; Issue: 1 Linguagem: Inglês

10.1016/j.cell.2007.11.042

1097-4172

Michelle M. Hill, Michele Bastiani, Robert Luetterforst, Matthew Kirkham, Annika Kirkham, Susan J. Nixon, Piers J. Walser, Daniel Abankwa, Viola Oorschot, Sally Martin, John F. Hancock, Robert G. Parton,

RNA Research and Splicing

Summary Caveolae are abundant cell-surface organelles involved in lipid regulation and endocytosis. We used comparative proteomics to identify PTRF (also called Cav-p60, Cavin) as a putative caveolar coat protein. PTRF-Cavin selectively associates with mature caveolae at the plasma membrane but not Golgi-localized caveolin. In prostate cancer PC3 cells, and during development of zebrafish notochord, lack of PTRF-Cavin expression correlates with lack of caveolae, and caveolin resides on flat plasma membrane. Expression of PTRF-Cavin in PC3 cells is sufficient to cause formation of caveolae. Knockdown of PTRF-Cavin reduces caveolae density, both in mammalian cells and in the zebrafish. Caveolin remains on the plasma membrane in PTRF-Cavin knockdown cells but exhibits increased lateral mobility and accelerated lysosomal degradation. We conclude that PTRF-Cavin is required for caveola formation and sequestration of mobile caveolin into immobile caveolae.