Produção Nacional
Revisado por pares
Biodiesel production via esterification reactions catalyzed by lipase
2006; National University of the South; Volume: 36; Issue: 4 Linguagem: Inglês
ISSN
1851-8796
AutoresAlessandra Paula Vieira, M. A. P. da Silva, Marta A. P. Langone,
Tópico(s)Microbial Metabolic Engineering and Bioproduction
ResumoThe synthesis of ethyl hexadecanoate was carried out by esterification of palmitic acid with ethanol in a solvent-free system. A commercial immobilized lipase (Lipozyme RM-IM) was mixed with the reagents, in a 15 mL closed batch reactor with constant stirring and coupled with a condenser. The effects of palmitic acid/ethanol molar ratio (0.16 to 1.84), reaction temperature (65 to 75 o C) and en- zyme concentration (0.48 to 5.52% w/w) on the ini- tial reaction rate of ethyl hexadecanoate were de- termined using central composite design 2 3 with six central points. Statistical analysis indicated that the enzyme concentration and palmitic acid/ethanol mo- lar ratio had been found to be the most significant variables affecting the initial reaction rate. The best result was obtained under the following experimen- tal conditions: palmitic acid/ethanol molar ratio of 0.50, temperature of 67 o C, and enzyme concentra- tion of 4.50% (w/w).
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