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Detection of HPV in oral rinse samples from OPSCC and non-OPSCC patients

2015; BioMed Central; Volume: 15; Issue: 1 Linguagem: Inglês

10.1186/s12903-015-0111-x

1472-6831

Juliet Dang, Qinghua Feng, Keith D. Eaton, Hona Jang, Nancy B. Kiviat,

Cleft Lip and Palate Research

Due to the increasing rates of oropharyngeal cancer, oral HPV infection is a significant concern. Methods for detecting oral HPVs is not standardized as there are different techniques available. We propose that use of oral rinse samples to detect for HPVs is a suitable technique within a clinic setting. Thus, our main objective is to study HPV detection in oral rinse samples. In our study, we used oral rinse sample collection coupled with real-time PCR to detect for HPVs types 16 and 18, and preferentially amplified FAP PCR samples to detect for a broad range of HPVs, in oropharyngeal squamous cell carcinoma (OPSCC), non-OPSCC, and healthy patients. Thirty three percent of 100 cancer patients were positive for any type of HPV; of those 23 were positive for HPV16. Only 1 of 110 healthy controls was positive (this subject was positive for HPV18). Our results indicate that HPV detection in oral rinse samples may be useful as a screening tool to detect HPV-associated oral cancers.