Large acceleration of α-chymotrypsin-catalyzed dipeptide formation by 18-crown-6 in organic solvents
1998; Wiley; Volume: 59; Issue: 5 Linguagem: Inglês
10.1002/(sici)1097-0290(19980905)59
ISSN1097-0290
AutoresDirk‐Jan van Unen, Johan F. J. Engbersen, David N. Reinhoudt,
Tópico(s)Molecular Sensors and Ion Detection
ResumoThe effects of 18-crown-6 on the synthesis of peptides catalyzed by alpha-chymotrypsin are reported. Lyophilization of the enzyme in the presence of 50 equivalents of 18-crown-6 results in a 425-fold enhanced activity when the reaction between the 2-chloroethylester of N-acetyl-L-phenylalanine and L-phenylalaninamide is carried out in acetonitrile. Addition of crown ether renders the dipeptide synthesis in nonaqueous solvents catalyzed by alpha-chymotrypsin possible on a preparative scale. The acceleration is observed in different solvents and for various peptide precursors. Copyright 1998 John Wiley & Sons, Inc.
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