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In Vitro Propagation of Arundinaria callosa Munro- an Edible Bamboo from Nodal Explants of Mature Plants

2009; Bentham Science Publishers; Volume: 3; Issue: 1 Linguagem: Inglês

10.2174/1874294700903010035

1874-2947

Waikhom Sayanika Devi, G. J. Sharma,

Plant Molecular Biology Research

A procedure for in vitro propagation of Arundinaria callosa Munro has been developed.The method allows bud-break from nodal segments containing single axillary bud on Murashige and Skoog (1962) medium supplemented with different concentrations of 6-benzylaminopurine (BAP).The early bud-break was obtained in 8.9-13.3M BAP, within 8-15 days.The position of the node on the culm of lateral branches also affected bud-break percentage and multiplication, mid-culm nodes are the most suitable.The optimal concentration of 13.3 M BAP is found significant for shoot multiplication.Addition of 1.0 M 3-indolebutyric acid (IBA) enhances the shoot multiplication rate.In vitro rooting was induced when 15 M IBA was incorporated for three subcultures in the shoot proliferation medium, was transferred to strength MS containing 25 M IBA and 0.05 M BAP, and finally withdrawn from the rooting medium.The regenerants were successfully transplanted into a soil mixture for acclimatization before field planting.