Apg‐2 has a chaperone‐like activity similar to Hsp110 and is overexpressed in hepatocellular carcinomas
2004; Wiley; Volume: 560; Issue: 1-3 Linguagem: Inglês
10.1016/s0014-5793(04)00034-1
ISSN1873-3468
AutoresKazuhisa Gotoh, Kohsuke Nonoguchi, Hiroaki Higashitsuji, Yoshiyuki Kaneko, Toshiharu Sakurai, Yasuhiko Sumitomo, Katsuhiko Itoh, John R. Subjeck, Jun Fujita,
Tópico(s)Protein Structure and Dynamics
ResumoHepatocellular carcinoma (HCC) is the fifth most common cancer in the world. We constructed subtracted cDNA libraries enriched with genes overexpressed in HCCs. Among the 17 genes identified were molecular chaperones, Hsp110, Hsp90B, and Hsp70‐1. Expression of the Hsp110 family members was further analyzed, and increased transcript levels of Hsp110 and Apg‐2, but not Apg‐1, were found in 12 and 14, respectively, of 18 HCCs. Immunohistochemical analysis demonstrated the overexpression of the proteins in tumor cells. Apg‐2 had chaperone ability similar to Hsp110 in a thermal denaturation assay using luciferase, and showed anti‐apoptotic activity. These results suggest that the Hsp110 family members play important roles in hepatocarcinogenesis through their chaperoning activities.
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