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Determined about sex: Sex-testing in 45 primate species using a 2Y/1X sex-typing assay

2014; Elsevier BV; Volume: 14; Linguagem: Inglês

10.1016/j.fsigen.2014.09.010

1878-0326

Valérie Choesmel Cadamuro, Caroline Bouakaze, Myriam Croze, Stéphanie Schiavinato, Laure Tonasso, Patrice Gérard, Jean‐Luc Fausser, Morgane Gibert, Jean‐Michel Dugoujon, José Braga, Patricia Balaresque,

Animal Behavior and Reproduction

Sex-testing using molecular genetic technique is routinely used in the fields of forensics, population genetics and conservation biology. However, none of the assay used so far allows a non-ambiguous and successful sex determination for human and non-human primate species. The most widely used method, AMELY/X, and its alternatives suffer from a set of drawbacks in humans and can rarely be used in New World primate species. Here, we designed a new sex-typing assay using a multiplexed PCR amplification of UTX and UTY-homologous loci and combined male-specific SRY locus. This method was successfully tested on 1048 samples, including 82 non-human primates from 45 Anthropoidea and Lemuriformes species and 966 human samples from 24 populations (Africans, Europeans, and South Americans). This sex-typing method is applicable across all primate species tested from Hominoidea to Indriidae, and also on various populations with different background origins; it represents a robust and cheap sex-typing assay to be used both by the anthropologist and primatologist communities.