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Integrin family of cell adhesion molecules in the injured brain: Regulation and cellular localization in the normal and regenerating mouse facial motor nucleus

1999; Wiley; Volume: 411; Issue: 1 Linguagem: Inglês

10.1002/(sici)1096-9861(19990816)411

1096-9861

Christian U.A. Kloss, Alexander Werner, Michael A. Klein, Jun Shen, Karen Menuz, J. Chistoph Probst, Georg W. Kreutzberg, Gennadij Raivich,

Barrier Structure and Function Studies

Journal of Comparative NeurologyVolume 411, Issue 1 p. 162-178 Integrin family of cell adhesion molecules in the injured brain: Regulation and cellular localization in the normal and regenerating mouse facial motor nucleus Christian U.A. Kloss, Christian U.A. Kloss Department of Neuromorphology, Max-Planck Institute for Neurobiology, D-82152 Martinsried, GermanySearch for more papers by this authorAlexander Werner, Alexander Werner Department of Neuromorphology, Max-Planck Institute for Neurobiology, D-82152 Martinsried, GermanySearch for more papers by this authorMichael A. Klein, Michael A. Klein Department of Neuromorphology, Max-Planck Institute for Neurobiology, D-82152 Martinsried, GermanySearch for more papers by this authorJun Shen, Jun Shen Department of Neuromorphology, Max-Planck Institute for Neurobiology, D-82152 Martinsried, GermanySearch for more papers by this authorKaren Menuz, Karen Menuz Department of Neuromorphology, Max-Planck Institute for Neurobiology, D-82152 Martinsried, GermanySearch for more papers by this authorJ. Chistoph Probst, J. Chistoph Probst Department of Neuromorphology, Max-Planck Institute for Neurobiology, D-82152 Martinsried, GermanySearch for more papers by this authorGeorg W. Kreutzberg, Georg W. Kreutzberg Department of Neuromorphology, Max-Planck Institute for Neurobiology, D-82152 Martinsried, GermanySearch for more papers by this authorGennadij Raivich, Corresponding Author Gennadij Raivich [email protected] Department of Neuromorphology, Max-Planck Institute for Neurobiology, D-82152 Martinsried, GermanyDepartment of Neuromorphology, Max-Planck-Institute for Neurobiology, D-82152 Martinsried, Germany.Search for more papers by this author Christian U.A. Kloss, Christian U.A. Kloss Department of Neuromorphology, Max-Planck Institute for Neurobiology, D-82152 Martinsried, GermanySearch for more papers by this authorAlexander Werner, Alexander Werner Department of Neuromorphology, Max-Planck Institute for Neurobiology, D-82152 Martinsried, GermanySearch for more papers by this authorMichael A. Klein, Michael A. Klein Department of Neuromorphology, Max-Planck Institute for Neurobiology, D-82152 Martinsried, GermanySearch for more papers by this authorJun Shen, Jun Shen Department of Neuromorphology, Max-Planck Institute for Neurobiology, D-82152 Martinsried, GermanySearch for more papers by this authorKaren Menuz, Karen Menuz Department of Neuromorphology, Max-Planck Institute for Neurobiology, D-82152 Martinsried, GermanySearch for more papers by this authorJ. Chistoph Probst, J. Chistoph Probst Department of Neuromorphology, Max-Planck Institute for Neurobiology, D-82152 Martinsried, GermanySearch for more papers by this authorGeorg W. Kreutzberg, Georg W. Kreutzberg Department of Neuromorphology, Max-Planck Institute for Neurobiology, D-82152 Martinsried, GermanySearch for more papers by this authorGennadij Raivich, Corresponding Author Gennadij Raivich [email protected] Department of Neuromorphology, Max-Planck Institute for Neurobiology, D-82152 Martinsried, GermanyDepartment of Neuromorphology, Max-Planck-Institute for Neurobiology, D-82152 Martinsried, Germany.Search for more papers by this author First published: 14 July 1999 https://doi.org/10.1002/(SICI)1096-9861(19990816)411:1 3.0.CO;2-WCitations: 100AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract Integrins are a large family of heterodimeric glycoproteins that play a crucial role in cell adhesion during development, inflammation, and tissue repair. In the current study, we investigated the localization of different integrin subunits in the mouse facial motor nucleus and their regulation after transection of the facial nerve. In the normal mouse brain, there was clear immunoreactivity for α5-, α6-, and β1-integrin subunits on blood vessel endothelia and for αM- and β2-subunits on resting parenchymal microglia. Facial nerve transection led to an up-regulation of the β1-subunit on the axotomized neurons and an increase in the α4-, α5-, α6-, β1-, αM-, αX-, and β2-subunits on the adjacent, activated microglia. Quantification of the microglial integrins revealed two different expression patterns. The subunits α5 and α6 showed a monophasic increase with a maximum at day 4, the αM-subunit a biphasic regulation, with an early peak at day 1 and an elevated plateau between day 14 and 42. At day 14, there was also an influx of lymphocytes immunoreactive for the α4β1- and αLβ2-integrins, which aggregated at sites of neural debris and phagocytotic microglia. This finding was accompanied by a significant increase of the α5β1-integrin on blood vessel endothelia. In summary, facial axotomy is followed by a strong and cell-type–specific expression of integrins on the affected neurons and on surrounding microglia, lymphocytes, and vascular endothelia. The presence of several, strikingly different temporal patterns suggests a selective involvement of these molecules in the different adhesive events during regeneration in the central nervous system. J. Comp. Neurol. 411:162–178, 1999. © 1999 Wiley-Liss, Inc. Citing Literature Volume411, Issue116 August 1999Pages 162-178 RelatedInformation