Expression of Orexin A and its Receptor 1 in the Epididymis of the South American Camelid Alpaca ( Vicugna pacos )
2013; Wiley; Volume: 43; Issue: 1 Linguagem: Inglês
10.1111/ahe.12046
ISSN1439-0264
AutoresGiovanna Liguori, Salvatore Paino, Nicola Mirabella, Caterina Squillacioti, A. De Luca, Alfredo Vittoria,
Tópico(s)Circadian rhythm and melatonin
ResumoAnatomia, Histologia, EmbryologiaVolume 43, Issue 1 p. 42-47 Original Article Expression of Orexin A and its Receptor 1 in the Epididymis of the South American Camelid Alpaca (Vicugna pacos) G. Liguori, G. Liguori Department of Biological Structures, Functions and Technologies, University of Naples Federico II, Via Veterinaria 1, I-80137 Naples, ItalySearch for more papers by this authorS. Paino, S. Paino Department of Animal Sciences, University of Basilicata, Via dell'Ateneo Lucano 10, I-85100 Potenza, ItalySearch for more papers by this authorN. Mirabella, N. Mirabella Department of Biological Structures, Functions and Technologies, University of Naples Federico II, Via Veterinaria 1, I-80137 Naples, ItalySearch for more papers by this authorC. Squillacioti, C. Squillacioti Department of Biological Structures, Functions and Technologies, University of Naples Federico II, Via Veterinaria 1, I-80137 Naples, ItalySearch for more papers by this authorA. De Luca, A. De Luca Department of Biological Structures, Functions and Technologies, University of Naples Federico II, Via Veterinaria 1, I-80137 Naples, ItalySearch for more papers by this authorA. Vittoria, Corresponding Author A. Vittoria Department of Biological Structures, Functions and Technologies, University of Naples Federico II, Via Veterinaria 1, I-80137 Naples, Italy Correspondence: Tel.: +39 0812536138; fax: +39 0812536097; e-mail: [email protected]Search for more papers by this author G. Liguori, G. Liguori Department of Biological Structures, Functions and Technologies, University of Naples Federico II, Via Veterinaria 1, I-80137 Naples, ItalySearch for more papers by this authorS. Paino, S. Paino Department of Animal Sciences, University of Basilicata, Via dell'Ateneo Lucano 10, I-85100 Potenza, ItalySearch for more papers by this authorN. Mirabella, N. Mirabella Department of Biological Structures, Functions and Technologies, University of Naples Federico II, Via Veterinaria 1, I-80137 Naples, ItalySearch for more papers by this authorC. Squillacioti, C. Squillacioti Department of Biological Structures, Functions and Technologies, University of Naples Federico II, Via Veterinaria 1, I-80137 Naples, ItalySearch for more papers by this authorA. De Luca, A. De Luca Department of Biological Structures, Functions and Technologies, University of Naples Federico II, Via Veterinaria 1, I-80137 Naples, ItalySearch for more papers by this authorA. Vittoria, Corresponding Author A. Vittoria Department of Biological Structures, Functions and Technologies, University of Naples Federico II, Via Veterinaria 1, I-80137 Naples, Italy Correspondence: Tel.: +39 0812536138; fax: +39 0812536097; e-mail: [email protected]Search for more papers by this author First published: 25 March 2013 https://doi.org/10.1111/ahe.12046Citations: 11 This work has been carried out at the Department of Biological Structures, Functions and Technologies, University of Naples Federico II, Naples (Italy). Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Summary Orexins A (ox A) and B are two peptides originally discovered in neurons of rat hypothalamus, and later found in different cellular types of the gastrointestinal and genital tracts. They arise from the proteolytic cleavage of a common precursor molecule, prepro-orexin, and bind to two receptors, namely receptor 1 (ox1r) and receptor 2 for orexins, that show different binding affinity. The central role of the two peptides has been extensively studied, whereas their activity in the periphery is still poorly known. Here, we investigated the presence of ox A and ox1r in the epididymis of a South American camelid species, the alpaca, by immunohistochemistry, and we also assessed the expression of prepro-orexin and ox1r in tissue extracts by Western blotting analysis. Ox A- and ox1r-immunoreactivity was found in the cytoplasm of principal cells of the caput epididymis. A prevalent supranuclear localization of granular-shaped positive material was observed. No positivity was present in the other cytotypes of epididymis. The expression of two peptides with molecular weight corresponding to those of prepro-orexin and ox1r, respectively, was detected in the tissue extracts from the organ. Citing Literature Volume43, Issue1February 2014Pages 42-47 RelatedInformation
Referência(s)