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Complement Fixation Reaction and Agar Gel Double Diffusion Test in Tyzzer's Disease of Mice

1967; Wiley; Volume: 11; Issue: 2 Linguagem: Inglês

10.1111/j.1348-0421.1967.tb00326.x

0021-5139

Kôsaku Fujiwara,

Viral Infections and Immunology Research

ABSTRACT Complement fixation reaction and agar gel double diffusion test were made using as antigens, water or phosphate buffer saline extracts of liver from mice severely infected with Tyzzer's disease. The supernatant of the extract heated at 56 C for 30 minutes was highly reactive with immune mouse serum in the complement fixation test, while an intense precipitate band was observed only with unhealed extracts in agar gel double diffusion tests. Cross reactions were observed in both tests between liver extracts from mice infected with different strains of organisms originating from different sources and showing varied degrees of virulence. Such reactions were not shown between Tyzzerimmune serum and liver extracts from uninfected or murine hepatitis‐infected mice, nor were they shown between the Tyzzer‐antigen and normal or anti‐murine hepatitis virus serum. The complement fixation test was used to test retired breeder mouse sera for disease contamination in mouse colonies. Positive reactions were observed in test sera collected from some breeding colonies that subsequently proved to be contaminated by the cortisone provocation test in young animals.