The human lymphocyte micronucleus assay
1984; Elsevier BV; Volume: 130; Issue: 6 Linguagem: Inglês
10.1016/0165-1161(84)90011-6
ISSN1878-7088
AutoresS.Z. Aghamohammadi, L.M. Henderson, R. J. Cole,
Tópico(s)DNA and Nucleic Acid Chemistry
ResumoThe induction of micronuclei in human cord blood lymphocytes by treatment with γ-irradiation and bleomycin has been measured. Culture durations which gave peak MN frequencies were determined. The lowest tested doses, 0.1 Gy irradiation and 1.25 μg/ml bleomycin, produced significant increases in the frequency of micronuclei. The spontaneous frequency of micronucleated lymphocytes in 28 cord blood samples ranged between 0.5 and 9.5 per thousand lymphocytes, with a modal value of 2.5. The method is evaluated for its potential usefulness in monitoring populations for chromosome breakage.
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