Crystal structure of a repair enzyme of oxidatively damaged DNA, MutM (Fpg), from an extreme thermophile, Thermus thermophilus HB8
2000; Springer Nature; Volume: 19; Issue: 15 Linguagem: Inglês
10.1093/emboj/19.15.3857
ISSN1460-2075
AutoresMitsuaki Sugahara, Tsutomu Mikawa, Takashi Kumasaka, Masaki Yamamoto, Ryuichi Kato, Keiichi Fukuyama, Yorinao Inoue, Seiki Kuramitsu,
Tópico(s)Enzyme Structure and Function
ResumoArticle1 August 2000free access Crystal structure of a repair enzyme of oxidatively damaged DNA, MutM (Fpg), from an extreme thermophile, Thermus thermophilus HB8 Mitsuaki Sugahara Mitsuaki Sugahara Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka, 560-0043 Japan RIKEN Harima Institute, SPring-8, 1-1-1 Kouto, Mikazuki, Sayo-gun, Hyogo, 679-5148 Japan Search for more papers by this author Tsutomu Mikawa Tsutomu Mikawa Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka, 560-0043 Japan Present address: Cellular and Molecular Biology Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama, 351-0198 Japan Search for more papers by this author Takashi Kumasaka Takashi Kumasaka RIKEN Harima Institute, SPring-8, 1-1-1 Kouto, Mikazuki, Sayo-gun, Hyogo, 679-5148 Japan Search for more papers by this author Masaki Yamamoto Masaki Yamamoto RIKEN Harima Institute, SPring-8, 1-1-1 Kouto, Mikazuki, Sayo-gun, Hyogo, 679-5148 Japan Search for more papers by this author Ryuichi Kato Ryuichi Kato Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka, 560-0043 Japan Search for more papers by this author Keiichi Fukuyama Keiichi Fukuyama Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka, 560-0043 Japan Search for more papers by this author Yorinao Inoue Yorinao Inoue RIKEN Harima Institute, SPring-8, 1-1-1 Kouto, Mikazuki, Sayo-gun, Hyogo, 679-5148 Japan Search for more papers by this author Seiki Kuramitsu Corresponding Author Seiki Kuramitsu Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka, 560-0043 Japan RIKEN Harima Institute, SPring-8, 1-1-1 Kouto, Mikazuki, Sayo-gun, Hyogo, 679-5148 Japan Genomic Sciences Center (RIKEN), 2-1 Hirosawa, Wako, Saitama, 351-0198 Japan Search for more papers by this author Mitsuaki Sugahara Mitsuaki Sugahara Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka, 560-0043 Japan RIKEN Harima Institute, SPring-8, 1-1-1 Kouto, Mikazuki, Sayo-gun, Hyogo, 679-5148 Japan Search for more papers by this author Tsutomu Mikawa Tsutomu Mikawa Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka, 560-0043 Japan Present address: Cellular and Molecular Biology Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama, 351-0198 Japan Search for more papers by this author Takashi Kumasaka Takashi Kumasaka RIKEN Harima Institute, SPring-8, 1-1-1 Kouto, Mikazuki, Sayo-gun, Hyogo, 679-5148 Japan Search for more papers by this author Masaki Yamamoto Masaki Yamamoto RIKEN Harima Institute, SPring-8, 1-1-1 Kouto, Mikazuki, Sayo-gun, Hyogo, 679-5148 Japan Search for more papers by this author Ryuichi Kato Ryuichi Kato Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka, 560-0043 Japan Search for more papers by this author Keiichi Fukuyama Keiichi Fukuyama Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka, 560-0043 Japan Search for more papers by this author Yorinao Inoue Yorinao Inoue RIKEN Harima Institute, SPring-8, 1-1-1 Kouto, Mikazuki, Sayo-gun, Hyogo, 679-5148 Japan Search for more papers by this author Seiki Kuramitsu Corresponding Author Seiki Kuramitsu Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka, 560-0043 Japan RIKEN Harima Institute, SPring-8, 1-1-1 Kouto, Mikazuki, Sayo-gun, Hyogo, 679-5148 Japan Genomic Sciences Center (RIKEN), 2-1 Hirosawa, Wako, Saitama, 351-0198 Japan Search for more papers by this author Author Information Mitsuaki Sugahara1,2, Tsutomu Mikawa1,3, Takashi Kumasaka2, Masaki Yamamoto2, Ryuichi Kato1, Keiichi Fukuyama1, Yorinao Inoue2 and Seiki Kuramitsu 1,2,4 1Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka, 560-0043 Japan 2RIKEN Harima Institute, SPring-8, 1-1-1 Kouto, Mikazuki, Sayo-gun, Hyogo, 679-5148 Japan 3Present address: Cellular and Molecular Biology Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama, 351-0198 Japan 4Genomic Sciences Center (RIKEN), 2-1 Hirosawa, Wako, Saitama, 351-0198 Japan *Corresponding author. E-mail: [email protected] The EMBO Journal (2000)19:3857-3869https://doi.org/10.1093/emboj/19.15.3857 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The MutM [formamidopyrimidine DNA glycosylase (Fpg)] protein is a trifunctional DNA base excision repair enzyme that removes a wide range of oxidatively damaged bases (N-glycosylase activity) and cleaves both the 3′- and 5′-phosphodiester bonds of the resulting apurinic/apyrimidinic site (AP lyase activity). The crystal structure of MutM from an extreme thermophile, Thermus thermophilus HB8, was determined at 1.9 Å resolution with multiwavelength anomalous diffraction phasing using the intrinsic Zn2+ ion of the zinc finger. MutM is composed of two distinct and novel domains connected by a flexible hinge. There is a large, electrostatically positive cleft lined by highly conserved residues between the domains. On the basis of the three-dimensional structure and taking account of previous biochemical experiments, we propose a DNA-binding mode and reaction mechanism for MutM. The locations of the putative catalytic residues and the two DNA-binding motifs (the zinc finger and the helix–two-turns–helix motifs) suggest that the oxidized base is flipped out from double-stranded DNA in the binding mode and excised by a catalytic mechanism similar to that of bifunctional base excision repair enzymes. Introduction In aerobic organisms, cellular DNA is frequently damaged by activated oxygen species from aerobic energy metabolism or oxidative stress. Highly reactive oxygen accelerates the rate of spontaneous mutation and has, therefore, been implicated as a causative agent for ageing and in the pathogenesis of disease, including cancer (Breimer, 1990; Ames et al., 1995). In particular, the 8-oxoguanine (GO) lesion is one of the most stable products of oxidative DNA damage (Dizdaroglu, 1985). GO can form a pair with adenine as well as with cytosine, resulting in a G:C to T:A transversion (Wood et al., 1990; Shibutani et al., 1991). In Escherichia coli, the GO repair system, composed of the MutM protein [EC 3.2.2.23], also called formamidopyrimidine DNA glycosylase (Fpg), accompanied by MutY and MutT, prevents this mutation (Michaels et al., 1992). The mutM (fpg) gene encoding the MutM protein is highly conserved across a wide range of aerobic bacteria. These enzymes (Mr 30 kDa) possess the invariant N-terminal sequence Pro-Glu-Leu-Pro-Glu-Val-, two strictly conserved lysine residues (Lys52 and Lys147) and a zinc finger motif (-Cys-X2-Cys-X16-Cys-X2-Cys-) at the C-terminus (Figure 1C). Figure 1.Overall structure, topology and amino acid sequence of MutM. (A) Stereo ribbon diagram and (B) topology of MutM. The MutM molecule consists of an N-terminal domain (residues 1–109, blue), a C-terminal domain [residues 131–203 (red and orange) and 238–266 (green)] and two long loops [110–130 and 204–237 (yellow)]. The N-terminal domain consists of a two-layered β-sandwich with two α-helices. The C-terminal domain consists of four α-helix bundles (red and orange) and a β-hairpin loop of the zinc finger motif (green). There are two conformers in an asymmetrical unit (see Figure 3B). The conformations of the two long loops (in yellow) in the interdomain cleft differ between the two conformers. These two long loops could work as a hinge in domain movement. Pro1 (shown by a ball-and-stick model), which works as a nucleophile in the glycosylase/AP lyase reaction, is situated at the bottom of the cleft. The highly conserved regions (purple) in the N-terminal domain, the H2TH motif (orange) and the zinc finger motif (green) are located around the cleft (see Figure 2A). (C) Aligned sequences of bacterial T.thermophilus MutM (Tt MutM) (Mikawa et al., 1998), E.coli MutM (Ec MutM) (Boiteux et al., 1987), A.thaliana MutM homologue 1 (At MutM H1) (Murphy and Gao, 1998; Ohtsubo et al., 1998) and E.coli endonuclease VIII (Ec EndoVIII) (Jiang et al., 1997). The α-helices (bars) and β-strands (arrows) are shown above the aligned sequences. The amino acid numbering refers to the T.thermophilus HB8 protein. Coloured letters (homologous residues in magenta, similar residues in green) represent the residues conserved among 16 MutM proteins (see also Figure 2A). The asterisks indicate every tenth amino acid residue of the T.thermophilus sequence. The highly conserved regions in the N-terminal domain (purple), the H2TH motif (orange) and the zinc finger motif (green) are boxed. Download figure Download PowerPoint MutM was originally isolated from E.coli and characterized as a DNA glycosylase that removes 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (Fapy), the imidazole ring-open form of N7-methylguanine (Boiteux et al., 1987). More recently, MutM was reported to bind to a wide range of oxidatively damaged bases (see Figure 5C), including GO paired to cytosine (Tchou et al., 1991), formamidopyrimidine (FapyG or FapyA) (Boiteux et al., 1992) and 5-hydroxycytosine (5OHC) (Hatahet et al., 1994) and to apurinic/apyrimidinic (AP) sites (Castaing et al., 1992). MutM has at least three enzymatic activities: (i) DNA glycosylase activity, which excises various damaged bases from DNA to produce an aldehydic abasic site; (ii) an AP lyase activity, which cleaves the 3′-phosphodiester bond at AP sites through β-elimination; and (iii) an alternative AP lyase activity, which cleaves the 5′-phosphodiester bond through δ-elimination (O'Connor et al., 1989; Boiteux et al., 1990; Bhagwat and Gerlt, 1996). In terms of the mechanism of MutM glycosylase/AP lyase activity, the initial step has been proposed to be formation of a covalent Schiff base intermediate by nucleophilic attack of the secondary amino group of Pro1 at the N-terminus on the deoxyribose C1′ of the damaged site (see Figure 7) (Tchou and Grollman, 1995; Bhagwat and Gerlt, 1996; Castaing et al., 1999). This is a common mechanism in other N-glycosylase/AP lyase base excision repair enzymes such as E.coli endonuclease III (EndoIII) (Kow and Wallace, 1987; Purmal et al., 1996) and bacteriophage T4 endonuclease V (T4 EndoV) (Schrock and Lloyd, 1991; Purmal et al., 1996). However, the activity of MutM N-glycosylase/AP lyase is quite distinct from those of EndoIII and T4 EndoV, which only cleave the 3′-phosphodiester bond via β-elimination. For EndoIII and T4 EndoV, the nucleophiles are the ϵ-amino group of Lys120 and the N-terminal α-amino group of Thr2, respectively, and the residues that activate the nucleophile and participate in the β-elimination reaction are Asp138 and Glu23, respectively. In the case of MutM, the Schiff base intermediate was trapped by hydration to identify the α-amino group of Pro1 as a nucleophile (Zharkov et al., 1997). Site-directed mutagenesis of the conserved Lys52 and Lys147 residues suggested their interaction with GO (Rabow and Kow, 1997; Sidorkina and Laval, 1998). It has also been suggested that the zinc finger motif is involved in the catalytic reaction (O'Connor et al., 1993; Tchou et al., 1993). However, the three-dimensional architecture of these residues in the N-glycosylation and β- and δ-elimination reactions remains to be elucidated. Figure 2.Conserved residues and electrostatic potential surface of MutM. (A) Stereo CPK model of MutM looking towards the interdomain large cleft. The surface is shown by a colour gradient according to residue conservation, with absolutely conserved residues in dark green. Homology was based on multiple alignment of the MutMs from T.thermophilus HB8 (Mikawa et al., 1998), E.coli (Boiteux et al., 1987), Neisseria meningitidis (Swartley and Stephens, 1995), Bacillus firmus (Ivey, 1990), Synechococcus elongatus naegeli (Floss et al., 1997), Bacillus subtilis (Lapidus et al., 1997), Salmonella typhimurium (Suzuki et al., 1997), Lactococcus lactis subsp. cremoris (Duwat et al., 1995), Haemophilus influenzae (sp|P44948), Synechocystis PCC6803 (sp|P74290), Mycobacterium leprae (emb|CAA19197.1), Mycobacterium tuberculosis (sp|Q10959), Streptococcus mutans (sp|P55045), Streptomyces coelicolor A3(2) (emb|CAB63194.1), Mycoplasma pneumoniae (sp|P75402) and Mycoplasma genitalium (sp|P55825). Homologous sequences were identified with PSI-BLAST (Altschul et al., 1997) and sequences were aligned with CLUSTAL W (Thompson et al., 1994). (B) The solvent-accessible surface is coloured according to electrostatic potential, with positive in blue and negative in red. The deep cleft between the two domains is electrostatically positive. Damaged DNA may bind to this large cleft. Download figure Download PowerPoint Figure 3.The model of the MutM–DNA complex and its hinge movement. (A) The model of the complex between the flipped out DNA and MutM in the closed form was obtained by molecular dynamics calculation. The viewing direction is similar to that in Figure 1A. The kinked DNA is drawn as a CPK model with backbones coloured in blue and with bases in grey. C and GO bases and their sugar residues before and after flipping out are shown by ball-and-stick models. All four conserved regions [(i) turns β5–β6 and β8–β9 in the sky-blue circle, (ii) the catalytic site in the pink circle, (iii) the H2TH motif in the yellow circle and (iv) the zinc finger motif in the light-green circle] are in the large cleft of the MutM molecule. The N-terminal domain can access the major groove of DNA and the zinc finger motif of the C-terminal domain can access the minor groove. The H2TH motif of the C-terminal domain is situated near the active site and may interact with the DNA backbone with the damaged base. (B) The superimposed structures of the two molecules in an asymmetrical unit. The superimposition was carried out to minimize the r.m.s.d. of the main chain in the C-terminal domain only. The main chain that deviates by >1 Å from the other molecule is only coloured red in the closed form monomer. Download figure Download PowerPoint The primary structures of bacterial (Thermus thermophilus HB8 and E.coli) MutM proteins are similar to those of MutM homologues in Arabidopsis thaliana (At MutM HI), a higher plant (Murphy et al., 1998; Ohtsubo et al., 1998), and E.coli endonuclease VIII (EndoVIII) (Jiang et al., 1997) (Figure 1C; the amino acid numbering refers to the T.thermophilus HB8 protein). These enzymes cleave both 3′- and 5′-phosphodiester bonds of the DNA strand by β- and δ-elimination, respectively, as does MutM protein (Melamede et al., 1994; Ohtsubo et al., 1998). However, AtMMH has no zinc finger motif and EndoVIII's substrate specificity is different from that of MutM. Recently, 8-oxoguanine DNA glycosylase 1 (OGG1) proteins from several eukaryotes, including humans, and ribosomal protein S3 from Drosophila melanogaster (Krokan et al., 1997) have been characterized as GO:C-specific N-glycosylase/AP lyases with activities identical to those of MutM. The crystal structures of several DNA glycosylases and their complexes with double-stranded DNA (dsDNA) have revealed common structural features, including a helix–hairpin–helix (HhH) motif and nucleotide flipping. The HhH motif, first reported in E.coli endonuclease III (EndoIII) (Thayer et al., 1995), has also been found in E.coli AlkA (Labahn et al., 1996; Yamagata et al., 1996; Hollis et al., 2000) and MutY (Guan et al., 1998) and in human OGG1 proteins (Bruner et al., 2000). Comparison of the amino acid sequence of MutM with those of these DNA glycosylases has not yet revealed common motifs or catalytic residues in MutM, despite their similar enzymatic properties. Here we report the X-ray crystallographic structure of the MutM enzyme from an extremely thermophilic bacterium, T.thermophilus HB8, determined at 1.9 Å resolution by multiwavelength anomalous diffraction (MAD) phasing of the Zn2+ ion. The crystal structure shows a zinc finger motif, a helix–two-turns–helix (H2TH) motif instead of an HhH motif and putative catalytic residues analogous to those of the other DNA glycosylases. We propose a model for the structure of the dsDNA–MutM complex. This model readily leads to a reaction mechanism for the enzyme and to an understanding of the catalytic reaction mechanism of related endonucleases. Results and discussion Overall structure MutM measures ∼50 × 30 × 30 Å3 and consists of two compact domains connected by a hinge region (Figure 1A). The N-terminal domain (residues 1–109, coloured blue) is a two-layered β-sandwich structure composed of nine antiparallel β-strands (β1–β9), except for strand β2, with an α-helix on each side of the β-sandwich (αA and αB) (Figure 1A and B). The C-terminal domain (residues 131–266, coloured red, orange or green) has four helices (αC–αF) and two β-strands (β10 and β11) that form a zinc finger motif (Figure 1A and B). There are two molecules in an asymmetrical unit in the crystal. The different overall conformations of these two molecules show that there is significant hinge movement, whereas the corresponding domains of the two molecules in an asymmetrical unit are substantially identical (Figure 3B). Figure 4.Two DNA-binding motifs in the MutM structure. (A) Superimposition of the MutM H2TH motif (purple) upon the HhH motif of E.coli EndoIII (green; PDB code 2ABK) (Thayer et al., 1995) and the H3TH motif of Pyrococcus furiosus Flap endonuclease-1 (FEN-1) (cyan; PDB code 1B43) (Hosfield et al., 1998). In the H2TH motif of MutM, there are two turns between α-helix D (αD) and α-helix E (αE). The amino acid sequences are aligned based on structural superimposition. The amino acid residues conserved among MutM, EndoIII and FEN-1 are coloured red. The HhH motif in EndoIII and the H3TH motif in FEN-1 are thought to interact with the dsDNA backbone (Doherty et al., 1996; Artymiuk et al., 1997). Therefore, the H2TH motif of MutM is presumed to play a similar role to the HhH and H3TH motifs and may contribute to non-sequence-specific recognition. (B) The ϵ-amino group of Lys147 forms hydrogen bonds (dashed lines) to the side chain of conserved residues and the carbonyl oxygen atoms of the main chain (ball-and-stick) at the interface of the H2TH motif (orange) and in the zinc finger motif (green). The decrease in catalytic efficiency caused by replacement of Lys147 with alanine (Rabow and Kow, 1997) can be attributed to the disruption of these hydrogen bonds and loss of folding integrity in this region of DNA interaction. Download figure Download PowerPoint MutM differs structurally from other base excision repair enzymes. A search of the protein structural database for the whole protein and for each domain showed no similar folding (the DALI server; Holm and Sander, 1993). Most of the conserved amino acid residues in this family of proteins are clustered in the large cleft between the two domains (Figures 1A and 2A). The putative essential residues for the enzymatic activities and the two DNA-binding motifs, the H2TH and zinc finger motifs, are located in this region. The N-terminal proline, whose α-amino group has been identified as a nucleophile in the DNA glycosylase/AP lyase reaction (Zharkov et al., 1997), occupies the bottom of the cleft (Figure 2A). In addition, the molecular surface inside the cleft is highly positive electrostatically (Figure 2B). Thus, the invariant residues in the cleft are candidates for the residues essential for DNA binding and catalysis. Figure 5.The structural basis for lesion recognition in MutM. (A) Model of recognition of GO:C-paired DNA (grey) by conserved amino acid residues (ball-and-stick) of MutM. (B) Schematic representation of the proposed lesion-recognition mechanism for the GO:C pair in dsDNA. Arg99 and Arg253 can interact with the keto groups of the GO and C base pair (blue) in both sides of dsDNA to disrupt the pairing hydrogen bonds in the model. The C8 oxo group of the GO base (red) in the DNA major groove could be pulled off by Met70 or Phe101 in the N-terminal domain. Phe101 could be inserted into the vacant site after nucleotide flipping to compensate for base stacking in the dsDNA. (C) Pairing of the oxidized bases in high- and low-efficiency MutM substrates is shown in the upper and lower rows, respectively (Hatahet et al., 1994; Tchou et al., 1994). Download figure Download PowerPoint From sequence comparison among the 16 MutM proteins, we propose the following four highly conserved regions involved in DNA binding and catalysis: (i) the invariant N-terminal six residues and the loop between β3 and β4 (coloured magenta in Figure 1A and B and the boxes coloured magenta in Figure 1C); (ii) the two loops between β5 and β6 and between β8 and β9 (coloured magenta); (iii) the H2TH motif (αD and αE, coloured orange), which resembles the HhH motif that interacts with the DNA phosphodiester backbone (Thayer et al., 1995; Mullen et al., 1997; Bruner et al., 2000; Hollis et al., 2000); and (iv) the zinc finger motif (β10 and β11, coloured green) forming a β-hairpin loop directed to the domain interface. MutM–DNA interactions A MutM–DNA footprinting experiment showed that MutM interacts with six nucleotides of the damaged strand, two nucleotides on the 3′ side of the lesion and three on the 5′ side (Castaing et al., 1999). In the enzymatic reaction, the α-imino group of Pro1 forms a Schiff base with C1′ of the deoxyribose in GO as a reaction intermediate (Zharkov et al., 1997). Lys52 has been suggested to interact with the C8 oxygen of the flipped GO base (Sidorkina et al., 1998). As mentioned above, there are two DNA-binding motifs in the MutM molecule, the H2TH and zinc finger motifs in the cleft. These regions are presumed to interact with dsDNA. Taking these results into account, we tried to construct the structure of the complex of MutM with dsDNA, using the molecule with the narrower cleft in an asymmetrical unit (Figure 3B), on the basis of several crystal structures of complexes of glycosylases and dsDNA. The structure selected as the initial model was calculated by molecular dynamics using GO-flipped dsDNA bent by 45°, as in the uracil-DNA-glycosylase–dsDNA complex (Parikh et al., 1998). The calculated model shows that the GO-flipped DNA fits nicely into the cleft of MutM (Figure 3). In the model complex, the DNA can interact with all four conserved regions mentioned above (Figure 1): the H2TH motif (yellow circle in Figure 3A), the zinc finger motif (light green circle), the two turns of β5–β6 and β8–β9 (sky-blue circle) and the putative catalytic site (pink circle). We will discuss the role of these regions further. H2TH motif The αD–αE region (residues 146–169) in the C-terminal domain has a characteristic H2TH motif structure with two turns between the two helices. The amino acid residues in the region are highly conserved among MutM homologues, including EndoVIII (Figure 1C). Similar consensus sequences are found in E.coli EndoIII (Thayer et al., 1995) and Pyrococcus furiosus Flap endonuclease-1 (FEN-1) (Hosfield et al., 1998) (Figure 4A). Figure 6.Architecture of the MutM active site. (A) The nucleophile Pro1 of MutM is surrounded by the invariant charged residues Glu2, Glu5 and Lys52, accompanied by several bound water molecules. The hydrophilic active site is very positive electrostatically (Figure 2B), so Lys52 is expected to be a proton donor for glycosidase activity in MutM. (B) Model of docking of the flipped out GO nucleotide to MutM active site in Figure 3A. The 8-oxo group of the GO base is located near the ϵ-amino group of Lys52, which as a proton donor may help the protonation of the oxo group and lead to depurination. (C) The putative reaction intermediate adduct with Pro1 after β-elimination, which is configurationally definitive due to its conjugated double bonding (Bhagwat and Gerlt, 1996), can fit into the active site and the hydroxyl group at C4′ of the opened deoxyribose reaches the carboxylic acid of Glu2, which is a good candidate for a proton acceptor. Download figure Download PowerPoint The HhH motif is found in E.coli EndoIII (Figure 4A, green) (Thayer et al., 1995) as a common non-specific DNA-binding motif and is implicated in binding to the DNA backbone phosphate. It is also observed in E.coli AlkA (Labahn et al., 1996; Yamagata et al., 1996; Hollis et al., 2000) and MutY (Guan et al., 1998) and in human OGG1 proteins (Bruner et al., 2000). These common HhH motifs in monofunctional glycosylases and bifunctional glycosylase/AP lyases suggest similar roles in DNA recognition, although the catalytic mechanisms of these enzymes are somewhat different (Labahn et al., 1996; Hollis et al., 2000). The helix–three-turns–helix (H3TH) motif, three turns between two helices, is found in P.furiosus FEN-1 (Figure 4A, cyan) (Hosfield et al., 1998). Both the HhH and H3TH motifs are found in a wide range of non-specific DNA-binding proteins, including nucleases, N-glycosylases, ligases, helicases, topoisomerases and polymerases, which are responsible for DNA synthesis or DNA repair. By analogy with the DNA polymerase β structure, it is suggested that each of these motifs binds to DNA in a non-specific manner by means of the formation of hydrogen bonds between amide nitrogen atoms of the protein backbone and phosphate oxygen atoms of the DNA backbone (Doherty et al., 1996; Artymiuk et al., 1997). Our model of the complex between MutM and damaged dsDNA (Figure 3A) shows that the H2TH motif can access the DNA backbone at the bottom of the cleft. Therefore, we conclude that the H2TH motif plays a similar role to the HhH and H3TH motifs, which participate in non-sequence-specific DNA recognition. Zinc finger motif MutM also has another DNA-binding motif, a zinc finger motif at the C-terminus (Figures 1, 3 and 4B). The zinc finger motif is a well known DNA-binding motif found in various sequence-specific DNA-binding proteins (O'Connor et al., 1993). The zinc finger motif of MutM (-Cys-X2-Cys-X16-Cys-X2-Cys-) is a simple β-hairpin loop that extends into the inter-domain cleft of MutM. Such a β-hairpin loop has also been reported in human 3-methyladenine DNA glycosylase (AAG) complexed with DNA (Lau et al., 1998), in which the tyrosine residue at the top of the hairpin loop interacts directly with the DNA lesion in the minor groove and may be essential for base-flipping. The [4Fe–4S] cluster loop motif of EndoIII also extends into the inter-domain cleft and is thought to intercalate into the minor groove of DNA (Thayer et al., 1995). The [4Fe–4S] cluster loop motif has a conserved basic residue at the edge of the loop. Thus, the positively charged Arg253 on the edge of the β-hairpin loop may interact with the minor groove of DNA in a specific manner similar to that of EndoIII, since the arginine residue is invariant among MutM family proteins (Figure 5A). Lys147 of MutM is a key structural residue in hydrogen bond networks between the H2TH motif and the zinc finger motif, maintaining the proper arrangement of the H2TH motif relative to the zinc finger motif (Figure 4B). Replacement of Lys147 by alanine causes a 50-fold decrease in the cleavage of GO:C substrates but does not affect AP site substrates (Rabow and Kow, 1997). The zinc finger motif, which specifically recognizes the GO:C pair, is firmly fixed with respect to the C-terminal domain by the hydrogen bond network, so the Lys147→Ala substitution would be expected to disrupt the interaction, resulting in a drastic decrease of activity against GO:C substrates. In contrast, the conformation of the H2TH motif at the core of the C-terminal domain, which is proposed to interact with the phosphate of the DNA backbone, would not be disturbed structurally by the mutation, and excision of the sugar moiety from the flipped out AP site would not be affected. Recognition of a wide range of oxidatively damaged bases MutM has broad specificity and high efficiency for oxidatively damaged lesions, including (i) GO paired to cytosine (Tchou et al., 1991); (ii) Fapy (Boiteux et al., 1992); and (iii) 5-hydroxycytosine (5OHC) paired to guanine (Hatahet et al., 1994) and (iv) AP sites (Castaing et al., 1992) (Figure 5C). The proposed model of the MutM complex (Figures 3 and 5A and B) supplies many clues for interpreting the specificity determinants of these substrates. The C8 oxygen of GO is located in the major groove of the model B-form dsDNA (Lipscomb et al., 1995) (Figure 5A and B). In the model, the damaged base interacts with turns β5–β6 and β8–β9 in the N-terminal domain, in which residues His67, Met70, Arg99 and Phe101 are conserved. These residues probably contribute to lesion recognition; in particular, Arg99 may form a hydrogen bond with the C6 oxo group at the damaged position. The minor groove of DNA interacts with the β-hairpin loop of the zinc finger motif in the model of the DNA complex. Arg253 is the only invariant residue in the β-hairpin loop; it can interact specifically with the C2 oxo group of cytosine in the GO:C pair (Figure 5B). The two arginine residues, Arg99 and Arg253, interact with both sides of the docked DNA to cleave the pairing hydrogen bonds between the GO:C bases. Substrates with low cleavage efficiency lack these oxo groups (Figure 5C) (Hatahet et al., 1994; Tchou et al., 1994). The C8 oxo group in the major groove may be pushed out by a large hydrophobic residue, either Met70 or Phe101, during binding of the dsDNA, using the hinge movement suggested by the existence of two molecules with different conformations in an asymmetrical unit (Figure 3
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