Portal de Periódicos da CAPES

A Genetically Encoded Photocaged Tyrosine

2006; Wiley; Volume: 45; Issue: 17 Linguagem: Inglês

10.1002/anie.200600264

1521-3773

Alexander Deiters, Dan Groff, Youngha Ryu, Jianming Xie, Peter G. Schultz,

Bacterial Genetics and Biotechnology

Angewandte Chemie International EditionVolume 45, Issue 17 p. 2728-2731 Communication A Genetically Encoded Photocaged Tyrosine† Alexander Deiters Prof., Alexander Deiters Prof. Department of Chemistry, North Carolina State University, Raleigh, NC 27695-8204, USA These authors contributed equally.Search for more papers by this authorDan Groff, Dan Groff Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA, Fax: (+1) 858-784-9440 These authors contributed equally.Search for more papers by this authorYoungha Ryu Dr., Youngha Ryu Dr. Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA, Fax: (+1) 858-784-9440Search for more papers by this authorJianming Xie, Jianming Xie Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA, Fax: (+1) 858-784-9440Search for more papers by this authorPeter G. Schultz Prof., Peter G. Schultz Prof. schultz@scripps.edu Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA, Fax: (+1) 858-784-9440Search for more papers by this author Alexander Deiters Prof., Alexander Deiters Prof. Department of Chemistry, North Carolina State University, Raleigh, NC 27695-8204, USA These authors contributed equally.Search for more papers by this authorDan Groff, Dan Groff Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA, Fax: (+1) 858-784-9440 These authors contributed equally.Search for more papers by this authorYoungha Ryu Dr., Youngha Ryu Dr. Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA, Fax: (+1) 858-784-9440Search for more papers by this authorJianming Xie, Jianming Xie Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA, Fax: (+1) 858-784-9440Search for more papers by this authorPeter G. Schultz Prof., Peter G. Schultz Prof. schultz@scripps.edu Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA, Fax: (+1) 858-784-9440Search for more papers by this author First published: 11 April 2006 https://doi.org/10.1002/anie.200600264Citations: 159 † A.D. gratefully acknowledges a postdoctoral fellowship from the Deutsche Forschungsgemeinschaft. This work was supported by funding from the National Institutes of Health (GM62159) and the Department of Energy (ER46051). This is manuscript 17952-CH of The Scripps Research Institute. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Activating proteins with light: A photocaged tyrosine was genetically encoded in E. coli in response to the amber codon TAG. Substitution of Tyr 503 in the active site of β-galactosidase allowed photoactivation of this enzyme in vitro or directly in bacteria with 360-nm light. This method should allow photoregulation of the activity of a variety of biological processes including transcription, signal transduction, and cellular trafficking. Citing Literature Supporting Information Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2006/z600264_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume45, Issue17April 21, 2006Pages 2728-2731 RelatedInformation