Polymerase Recognition of Unnatural Base Pairs
2002; Wiley; Volume: 41; Issue: 20 Linguagem: Inglês
10.1002/1521-3773(20021018)41
ISSN1521-3773
AutoresChengzhi Yu, Allison A. Henry, Floyd E. Romesberg, Peter G. Schultz,
Tópico(s)Molecular Biology Techniques and Applications
ResumoAngewandte Chemie International EditionVolume 41, Issue 20 p. 3841-3844 Communication Polymerase Recognition of Unnatural Base Pairs† Chengzhi Yu Dr., Chengzhi Yu Dr. Department of Chemistry The Scripps Research Institute 10550 N. Torrey Pines Road, La Jolla, CA 92037 (USA) Fax: (+1) 858-784-7472Search for more papers by this authorAllison A. Henry, Allison A. Henry Department of Chemistry The Scripps Research Institute 10550 N. Torrey Pines Road, La Jolla, CA 92037 (USA) Fax: (+1) 858-784-7472Search for more papers by this authorFloyd E. Romesberg Prof., Floyd E. Romesberg Prof. [email protected] Department of Chemistry The Scripps Research Institute 10550 N. Torrey Pines Road, La Jolla, CA 92037 (USA) Fax: (+1) 858-784-7472Search for more papers by this authorPeter G. Schultz Prof., Peter G. Schultz Prof. [email protected] Department of Chemistry The Scripps Research Institute 10550 N. Torrey Pines Road, La Jolla, CA 92037 (USA) Fax: (+1) 858-784-7472Search for more papers by this author Chengzhi Yu Dr., Chengzhi Yu Dr. Department of Chemistry The Scripps Research Institute 10550 N. Torrey Pines Road, La Jolla, CA 92037 (USA) Fax: (+1) 858-784-7472Search for more papers by this authorAllison A. Henry, Allison A. Henry Department of Chemistry The Scripps Research Institute 10550 N. Torrey Pines Road, La Jolla, CA 92037 (USA) Fax: (+1) 858-784-7472Search for more papers by this authorFloyd E. Romesberg Prof., Floyd E. Romesberg Prof. [email protected] Department of Chemistry The Scripps Research Institute 10550 N. Torrey Pines Road, La Jolla, CA 92037 (USA) Fax: (+1) 858-784-7472Search for more papers by this authorPeter G. Schultz Prof., Peter G. Schultz Prof. [email protected] Department of Chemistry The Scripps Research Institute 10550 N. Torrey Pines Road, La Jolla, CA 92037 (USA) Fax: (+1) 858-784-7472Search for more papers by this author First published: 18 October 2002 https://doi.org/10.1002/1521-3773(20021018)41:20 3.0.CO;2-QCitations: 49 † This work was funded by the National Institutes of Health (GM 60005 to F.E.R) and by the Skaggs Institute for Chemical Biology (F.E.R. and P.G.S.) Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Graphical Abstract More efficient and selective synthesis as well as more efficient extension by a DNA polymerase is achieved for unnatural base pairs upon heteroatom substitution. This has been demonstrated with modification of ICS (structure shown): Replacement of C6 with nitrogen and thio substitution at C10 provides the base SNICS, which forms stable self-pairs and can therefore be used for efficient unnatural base pair replication. Supporting Information Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2002/z18596_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. References 1E. T. Kool, Annu. Rev. Biophys. Biomol. 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