Agrobacterium tumefaciens: a natural tool for plant transformation
1998; Elsevier BV; Volume: 1; Issue: 3 Linguagem: Inglês
10.4067/s0717-34581998000300002
ISSN0717-3458
AutoresG Riva, Joel González‐Cabrera, Roberto I. Vázquez-Padrón, Camilo Ayra–Pardo,
Tópico(s)Chromosomal and Genetic Variations
ResumoUpdated information of mechanisms for T-DNA transfer to plant cells by Agrobacterium tumefaciens is provided, focused on the role played by the different components of the virulence system. The general assessments for the establishment of efficient transformation protocols are discussed with an emphasis in the application of this methodology to monocotyledonous plants. Based on our own experience, we present the establishment of sugarcane transformation by A. tumefaciens as a model of application of this methodology to an important culture plant species, previously considered recalcitrant and inaccessible for this type of genetic manipulation.
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