Isolation and characterization of the outer membrane of Acinetobacter calcoaceticus
1984; Elsevier BV; Volume: 1; Issue: 2 Linguagem: Inglês
10.1016/0168-1656(84)90013-0
ISSN1873-4863
AutoresB. Fischer, R. Claus, H.‐P. Kleber,
Tópico(s)Enzyme Production and Characterization
ResumoA method for the separation of the outer membrane (OM) from the cytoplasmic membrane (CM) of Acinetobacter calcoaceticus 69/V grown on different carbon sources is described. The contamination of the OM with CM was less than 10%. Independent of the carbon source, five protein bands with apparent molecular weights of 47 000, 33000, 21 000, 19 000 and 12 000 were found by solubilization at 37°C and six bands at 100°C (apparent Mr 53 000, 47 000, 38 000, 26 000, 21000, 12000). Three proteins were modifiable by heat. With the periodic acid-Schiff procedure the bands with apparent Mr of 33 000 and 12 000 were made visible. After growth on d,l-carnitine an additional two non-heat-modifiable protein bands with apparent Mr between 40 000 and 45 000 were detected. By cultivation on acetate and peptone as carbon source one additional band (Mr 15 000) from OM of cells could be found.
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