hTERT gene dosage correlates with telomerase activity in human lung cancer cell lines
2002; Elsevier BV; Volume: 176; Issue: 1 Linguagem: Inglês
10.1016/s0304-3835(01)00644-9
ISSN1872-7980
AutoresGabriele Saretzki, Simone Petersen, Iver Petersen, Konrad Kölble, Thomas von Zglinicki,
Tópico(s)Biosensors and Analytical Detection
ResumoMaintenance of telomeres, most often by telomerase, is a necessary prerequisite for immortality of eukaryotic cells. To better understand the mechanisms of telomerase up-regulation during tumorigenesis, we analysed the gene dosage of hTERT on chromosome 5p15, a region known to be overrepresented in a variety of malignancies, in 20 lung cancer cell lines by Southern blotting, fluorescence in-situ hybridization, and comparative genomic hybridization. We found a significant correlation between hTERT gene dosage, hTERT mRNA expression and telomerase activity. Imbalances of chromosome 5p may exert functionally relevant hTERT gene dosage effects in human lung cancer.
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