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Hybrid selection for sequencing pathogen genomes from clinical samples

2011; BioMed Central; Volume: 12; Issue: 8 Linguagem: Inglês

10.1186/gb-2011-12-8-r73

1474-760X

Alexandre Melnikov, Kevin Galinsky, Peter Rogov, Timothy R. Fennell, Daria Van Tyne, Carsten Russ, Rachel F. Daniels, Kayla G. Barnes, James Bochicchio, Daouda Ndiaye, Papa Diogoye Séne, Dyann F. Wirth, Chad Nusbaum, Sarah K. Volkman, Bruce W. Birren, Andreas Gnirke, Daniel E. Neafsey,

Parasitic Diseases Research and Treatment

We have adapted a solution hybrid selection protocol to enrich pathogen DNA in clinical samples dominated by human genetic material. Using mock mixtures of human and Plasmodium falciparum malaria parasite DNA as well as clinical samples from infected patients, we demonstrate an average of approximately 40-fold enrichment of parasite DNA after hybrid selection. This approach will enable efficient genome sequencing of pathogens from clinical samples, as well as sequencing of endosymbiotic organisms such as Wolbachia that live inside diverse metazoan phyla.