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Response of the Microaerophilic Bifidobacterium Species, B. boum and B. thermophilum , to Oxygen

2006; American Society for Microbiology; Volume: 72; Issue: 10 Linguagem: Inglês

10.1128/aem.01216-06

1098-5336

Shinji Kawasaki, Tsuyoshi Mimura, Takumi Satoh, Kouji Takeda, Youichi Niimura,

Hemoglobin structure and function

ABSTRACT We investigated the effects of O 2 on Bifidobacterium species using liquid shaking cultures under various O 2 concentrations. Although most of the Bifidobacterium species we selected showed O 2 sensitivity, two species, B. boum and B. thermophilum , demonstrated microaerophilic profiles. The growth of B. bifidum and B. longum was inhibited under high-O 2 conditions accompanied by the accumulation of H 2 O 2 in the medium, and growth was restored by adding catalase to the medium. B. boum and B. thermophilum grew well even under 20% O 2 conditions without H 2 O 2 accumulation, and growth was stimulated compared to anoxic growth. H 2 O-forming NADH oxidase activities were detected dominantly in cell extracts of B. boum and B. thermophilum under acidic reaction conditions (pH 5.0 to 6.0).