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Increased Detection of Respiratory Syncytial Virus, Influenza Viruses, Parainfluenza Viruses, and Adenoviruses with Real-Time PCR in Samples from Patients with Respiratory Symptoms

2007; American Society for Microbiology; Volume: 45; Issue: 7 Linguagem: Inglês

10.1128/jcm.00848-07

1098-660X

Alma C. van de Pol, Anton M. van Loon, Tom F.W. Wolfs, Nicolaas J. G. Jansen, Monique Nijhuis, Els Klein Breteler, Rob Schuurman, John W. A. Rossen,

Influenza Virus Research Studies

Respiratory samples (n = 267) from hospitalized patients with respiratory symptoms were tested by real-time PCR, viral culture, and direct immunofluorescence for respiratory syncytial virus, influenza virus, parainfluenza viruses, and adenoviruses. Compared with conventional diagnostic tests, real-time PCR increased the diagnostic yields for these viruses from 24% to 43% and from 3.5% to 36% for children and adults, respectively.