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Immediate Detection of Living Bacteria at Ultralow Concentrations Using a Carbon Nanotube Based Potentiometric Aptasensor

2009; Wiley; Volume: 48; Issue: 40 Linguagem: Inglês

10.1002/anie.200902090

1521-3773

Gustavo A. Zelada‐Guillén, Jordi Riu, Ali Düzgün, F. Xavier Rius,

Electrochemical Analysis and Applications

Angewandte Chemie International EditionVolume 48, Issue 40 p. 7334-7337 Communication Immediate Detection of Living Bacteria at Ultralow Concentrations Using a Carbon Nanotube Based Potentiometric Aptasensor† Gustavo A. Zelada-Guillén, Gustavo A. Zelada-Guillén Departament de Química Analítica i Química Orgànica, Universitat Rovira i Virgili, 43007 Tarragona (Spain)Search for more papers by this authorJordi Riu, Jordi Riu [email protected] Departament de Química Analítica i Química Orgànica, Universitat Rovira i Virgili, 43007 Tarragona (Spain)Search for more papers by this authorAli Düzgün, Ali Düzgün Departament de Química Analítica i Química Orgànica, Universitat Rovira i Virgili, 43007 Tarragona (Spain)Search for more papers by this authorF. Xavier Rius, F. Xavier Rius [email protected] Departament de Química Analítica i Química Orgànica, Universitat Rovira i Virgili, 43007 Tarragona (Spain)Search for more papers by this author Gustavo A. Zelada-Guillén, Gustavo A. Zelada-Guillén Departament de Química Analítica i Química Orgànica, Universitat Rovira i Virgili, 43007 Tarragona (Spain)Search for more papers by this authorJordi Riu, Jordi Riu [email protected] Departament de Química Analítica i Química Orgànica, Universitat Rovira i Virgili, 43007 Tarragona (Spain)Search for more papers by this authorAli Düzgün, Ali Düzgün Departament de Química Analítica i Química Orgànica, Universitat Rovira i Virgili, 43007 Tarragona (Spain)Search for more papers by this authorF. Xavier Rius, F. Xavier Rius [email protected] Departament de Química Analítica i Química Orgànica, Universitat Rovira i Virgili, 43007 Tarragona (Spain)Search for more papers by this author First published: 17 September 2009 https://doi.org/10.1002/anie.200902090Citations: 234 † We would like to thank A. Maroto and R. Villamizar for their helpful comments and also S. Moreno for the loan of essential equipment. This study has been supported by the Spanish Ministry of Education and Science, MEC, through the project grants NAN2004-09306-C05-05 and CTQ2007-67570/BQU. G.A.Z.-G. would like to thank the Generalitat de Catalunya (Catalan Government) and European Social Fund for awarding the FI DGR fellowship. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Graphical Abstract Keeping the doctor away: An aptamer attached to an electrode coated with single-walled carbon nanotubes interacts selectively with bacteria (see picture). The resulting electrochemical response is highly accurate and reproducible and starts at ultralow bacteria concentrations, thus providing a simple, selective method for pathogen detection. Supporting Information Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Filename Description anie_200902090_sm_miscellaneous_information.pdf103.9 KB miscellaneous_information Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. References 1C. A. Batt, Science 2007, 316, 1579. 2P. Belgrader, W. Benett, D. Hadley, J. Richards, P. Stratton, R. Mariella, Jr., F. Milanovich, Science 1999, 284, 449. 3Y. Li, Y. T. H. Cu, D. Luo, Nat. Biotechnol. 2005, 23, 885. 4B. Ilic, D. Czaplewski, M. Zalalutdinov, H. G. Craighead, P. Neuzil, C. Campagnolo, C. Batt, J. Vac. Sci. Technol. B 2001, 19, 2825. 5H. Gu, K. Xu, C. Xu, B. Xu, Chem. Commun. 2006, 941. 6T. H. Rider, M. S. Petrovick, F. E. Nargi, J. D. Harper, E. D. Schwoebel, R. H. Mathews, D. J. Blanchard, L. T. Bortolin, A. M. Young, J. Chen, M. A. Hollis, Science 2003, 301, 213. 7E. Bakker, E. Pretsch, Angew. Chem. 2007, 119, 5758; Angew. Chem. Int. Ed. 2007, 46, 5660. 8G. A. Crespo, S. Macho, F. X. Rius, Anal. Chem. 2008, 80, 1316. 9H. Dai, Acc. Chem. Res. 2002, 35, 1035. 10G. A. Crespo, S. Macho, J. Bobacka, F. X. Rius, Anal. Chem. 2009, 81, 676. 11L. Agüí, P. Yáñez-Sedeño, J. M. Pingarrón, Anal. Chim. Acta 2008, 622, 11. 12S. M. Shamah, J. M. Healy, S. T. Cload, Acc. Chem. Res. 2008, 41, 130. 13S. Tombelli, M. Minunni, M. Mascini, Biosens. Bioelectron. 2005, 20, 2424. 14N. de-los-Santos-Álvarez, M. J. Lobo-Castañón, A. J. Miranda-Ordieres, P. Tuñón-Blanco, TrAC Trends Anal. Chem. 2008, 27, 437. 15M. Zheng, A. Jagota, M. S. Strano, A. P. Santos, P. Barone, S. G. Chou, B. A. Diner, M. S. Dresselhaus, R. S. Mclean, G. B. Onoa, G. G. Samsonidze, E. D. Semke, M. Usrey, D. J. Walls, Science 2003, 302, 1545. 16H. M. So, D. W. Park, E. K. Jeon, Y. H. Kim, B. S. Kim, C. K. Lee, S. Y. Choi, S. C. Kim, H. Chang, J. O. Lee, Small 2008, 4, 197. 17A. Star, E. Tu, J. Niemann, J. C. P. Gabriel, C. S. Joiner, C. Valcke, Proc. Natl. Acad. Sci. USA 2006, 103, 921. 18Q. Pan, X. L. Zhang, H. Y. Wu, P. W. He, F. Wang, M. S. Zhang, J. M. Hu, B. Xia, J. Wu, Antimicrob. Agents Chemother. 2005, 49, 4052. 19C. A. Furtado, U. J. Kim, H. R. Gutierrez, L. Pan, E. C. Dickey, P. C. Eklund, J. Am. Chem. Soc. 2004, 126, 6095. 20S. S. Wong, E. Joselevich, A. T. Woolley, C. L. Cheung, C. M. Lieber, Nature 1998, 394, 52. 21D. H. Jung, B. H. Kim, Y. K. Ko, M. S. Jung, S. Jung, S. Y. Lee, H. T. Jung, Langmuir 2004, 20, 8886. 22R. R. Johnson, A. T. C. Johnson, M. L. Klein, Nano Lett. 2008, 8, 69. 23Y. Hong, D. G. Brown, Langmuir 2008, 24, 5003. 24L. A. Currie, Pure Appl. Chem. 1995, 67, 1699. Citing Literature Volume48, Issue40September 21, 2009Pages 7334-7337 ReferencesRelatedInformation