Portal de Periódicos da CAPES

Revisão Produção Nacional Revisado por pares

BIBTEX RIS

Methods for obtaining reliable and reproducible results in studies of C andida biofilms formed in vitro

2013; Wiley; Volume: 56; Issue: 6 Linguagem: Inglês

10.1111/myc.12092

1439-0507

Anna Carolina Borges Pereira Costa, Cristiane Aparecida Pereira, Fernanda Freire, Juliana Campos Junqueira, Antônio Olavo Cardoso Jorge,

Antimicrobial agents and applications

Summary Biofilm formation is one of the most important attributes for virulence in C andida species and contributes to increased resistance to antifungal drugs and host immune mechanisms. These features have led to the development of several methodologies to reproduce a sessile community in vitro that can be used to study the development of a biofilm, its interaction with other microorganisms and the environment, and its susceptibility to available antifungal agents and also to search for new therapy strategies. The purpose of this review is to describe the most commonly used methods to study C andida biofilms in vitro , to discuss the benefits and limitations of the different methods to induce biofilm formation, and to analyse the architecture, viability and growth kinetics of C andida biofilms.