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CHANGES IN REGIONAL RENAL BLOOD FLOW AFTER UNILATERAL NEPHRECTOMY USING THE TECHNIQUES OF AUTORADIOGRAPHY AND MICROAUTORADIOGRAPHY

1998; Lippincott Williams & Wilkins; Volume: 160; Issue: 3 Part 1 Linguagem: Inglês

10.1016/s0022-5347(01)62834-9

1527-3792

Leonie S. Young, Mark Regan, Paul Sweeney, Kevin M. Barry, Michael P. Ryan, John M. Fitzpatrick,

Urinary Tract Infections Management

No AccessJournal of UrologyInvestigative Urology1 Sep 1998CHANGES IN REGIONAL RENAL BLOOD FLOW AFTER UNILATERAL NEPHRECTOMY USING THE TECHNIQUES OF AUTORADIOGRAPHY AND MICROAUTORADIOGRAPHY LEONIE S. YOUNG, MARK C. REGAN, PAUL SWEENEY, KEVIN M. BARRY, MICHAEL P. RYAN, and JOHN M. FITZPATRICK LEONIE S. YOUNGLEONIE S. YOUNG More articles by this author , MARK C. REGANMARK C. REGAN More articles by this author , PAUL SWEENEYPAUL SWEENEY More articles by this author , KEVIN M. BARRYKEVIN M. BARRY More articles by this author , MICHAEL P. RYANMICHAEL P. RYAN More articles by this author , and JOHN M. FITZPATRICKJOHN M. FITZPATRICK More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)62834-9AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: To determine alterations in regional renal blood flow following unilateral nephrectomy using an autoradiographic technique. The role of prostaglandins and the sympathetic nervous system in the mediation of these changes was assessed. Materials and Methods: C-14 iodoantipyrine was used as a tracer to measure intrarenal blood flow in anaesthetised rats at multiple time points following nephrectomy. Autoradiographs were produced from tissue sections. C-14 concentrations were measured from standards thus allowing blood flow values to be calculated. Results: Base line values for cortical and medullary blood flow were 806 +/- 63 and 373 +/- 39 ml./100 gm./min. (mean +/- SEM) respectively. At 2 hours post nephrectomy blood flow to both the cortex and medulla increased significantly (1152 +/- 54 and 594 +/- 37; p <0.05). Blood flow had returned to control levels by 24 hours and was maintained at 5 days post-nephrectomy. Multiple discrete regions of high blood flow within the cortex were observed. Microautoradiography defined the morphological location of these discrete regions of higher blood flow as periglomerular vasculature. Diclofenac administration did not inhibit the augmentation in cortical blood flow post-nephrectomy, while medullary blood flow fell below base line values at both 30 minutes and 2 hours following nephrectomy. Sympathetic denervation did not affect the changes in cortical blood flow seen following nephrectomy, but did ameliorate the changes in medullary blood flow. Conclusions: Significant, transient changes in regional renal blood flow occur in the residual kidney following unilateral nephrectomy. The interaction between vasoactive mediators and the autonomic nervous system which produces changes in cortical blood flow is complex. It is evident, however, that medullary blood flow is dependent on local prostaglandin production and is also influenced by sympathetic nervous supply. References 1 : Autoregulation of blood flow in the rat kidney. Am. J. Physiol.1975; 228: 127. Google Scholar 2 : Renal haemodynamics. Am. J. Med.1964; 36: 698. Google Scholar 3 : Visualisation of renal autoregulation in the split hydronephrotic kidneys of the rat. Kidney Int.1989; 35: 1151. Google Scholar 8 : A study of regional distribution of renal blood flow using quantitative autoradiography. Am. J. Physiol.1992; 263: 958. Google Scholar 9 : Influence of prostaglandins on papillary blood flow and pressure naturetic response. Hypertension1990; 15: 29. Google Scholar 10 : Cine-computed tomography assessment of regional renal blood flow. Acta Radiol.1989; 31: 77. 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Google Scholar From the University Department of Surgery and Pharmacology, Mater Misericordae Hospital and University College Dublin, Dublin, Ireland© 1998 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited bySprenkle P, Wren J, Maschino A, Feifer A, Power N, Ghoneim T, Sternberg I, Fleisher M and Russo P (2018) Urine Neutrophil Gelatinase-Associated Lipocalin as a Marker of Acute Kidney Injury After Kidney SurgeryJournal of Urology, VOL. 190, NO. 1, (159-164), Online publication date: 1-Jul-2013.HEGARTY N, YOUNG L, O’NEILL A, WATSON R and FITZPATRICK J (2018) Endothelin in Unilateral Ureteral Obstruction: Vascular and Cellular EffectsJournal of Urology, VOL. 169, NO. 2, (740-744), Online publication date: 1-Feb-2003. Volume 160Issue 3 Part 1September 1998Page: 926-931 Advertisement Copyright & Permissions© 1998 by American Urological Association, Inc.MetricsAuthor Information LEONIE S. YOUNG More articles by this author MARK C. REGAN More articles by this author PAUL SWEENEY More articles by this author KEVIN M. BARRY More articles by this author MICHAEL P. RYAN More articles by this author JOHN M. FITZPATRICK More articles by this author Expand All Advertisement PDF downloadLoading ...