Portal de Periódicos da CAPES

Microvesicles from Sickle Erythrocytes and their Relation to Irreversible Sickling

1981; Wiley; Volume: 47; Issue: 3 Linguagem: Inglês

10.1111/j.1365-2141.1981.tb02805.x

1365-2141

David Allan, A. R. Limbrick, Paul Thomas, Maxwell P. Westerman,

Neonatal Health and Biochemistry

S ummary . Incubation of sickle (HbS) erythrocytes for periods up to 96 h leads to the formation of irreversibly sickled cells (ISCs) and to the release of spectrin‐free microvesicles similar to those derived from aged or Ca 2+ ‐ionophore‐treated normal erythrocytes. The sickle microvesicles were somewhat larger than those from normal cells and showed minor differences in their membrane polypeptide composition. Sickle microvesicles were no different from their parent cells in their content of fetal haemoglobin. Neither microvesiculation nor formation of irreversibly sickled cells required the presence of Ca 2+ in the medium but Ca 2+ did accelerate both processes. Although in these prolonged incubations microvesiculation appeared to occur concomitantly with the formation of ISCs, it is not clear whether or not microvesiculation is a necessary prelude to irreversible sickling.