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BIBTEX RIS

Characterization of Aspergillus niger B-1 RNase and Its Inhibitory Effect on Pollen Germination and Pollen Tube Growth in Selected Tree Fruit

2000; American Society for Horticultural Science; Volume: 125; Issue: 1 Linguagem: Inglês

10.21273/jashs.125.1.9

ISSN

2327-9788

Autores

Levava Roiz, Uzi Ozeri, R. Gören, Oded Shoseyov,

Tópico(s)

Plant and animal studies

Resumo

Aspergillus niger B-1 (CMI CC 324626) extracellular RNase (RNase B1) was purified to homogeneity. It was found to contain two isoforms of 32- and 40-kDa glycoproteins, sharing a 29-kDa protein moiety. Optimal RNase activity was observed at 60 °C and pH 3.5. In `Almog' peach [ Prunus persica (L.) Batsch (Peach Group) `Almog'] and `Murcott' tangerine ( Citrus reticulata Blanco `Murcott') the enzyme inhibited pollen germination and pollen tube growth in vitro as well as in vivo. In field experiments, spray application of the RNase caused a reduction in `Fantasia' nectarine [ Prunus persica (L.) Batsch (Nectarine Group) `Fantasia'] fruit set and interfered with embryo development. The biological effect of the RNase may be of horticultural value, due to its potential to control fertilization.

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