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Contact‐Mediated Quenching for RNA Imaging in Bacteria with a Fluorophore‐Binding Aptamer

2013; Wiley; Volume: 52; Issue: 50 Linguagem: Inglês

10.1002/anie.201306622

1521-3773

Murat Sünbül, Andres Jäschke,

DNA and Nucleic Acid Chemistry

Bind and shine: Sulforhodamine B was converted into a very efficient fluorescence turn-on probe exclusively by contact quenching. The fluorescence of the probe increases more than 100-fold upon binding to a sulforhodamine-binding RNA aptamer (SRB-2). The probe and the SRB-2 aptamer were used to monitor transcriptions in real-time and to image RNA in live bacteria. As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.