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Expression of Nitric Oxide Synthase Immunoreactivity in the Human Female Intramural Striated Urethral Sphincter

2003; Lippincott Williams & Wilkins; Volume: 169; Issue: 6 Linguagem: Inglês

10.1097/01.ju.0000055607.67581.09

1527-3792

Kossen M.T. Ho, Mario Cortina‐Borja, Katarina Persson, Alison F. Brading, Karl‐Erik Andersson,

Nitric Oxide and Endothelin Effects

No AccessJournal of UrologyINVESTIGATIVE UROLOGY1 Jun 2003Expression of Nitric Oxide Synthase Immunoreactivity in the Human Female Intramural Striated Urethral Sphincter KOSSEN M.T. HO, MARIO CORTINA BORJA, KATARINA PERSSON, ALISON F. BRADING, and KARL-ERIK ANDERSSON KOSSEN M.T. HOKOSSEN M.T. HO , MARIO CORTINA BORJAMARIO CORTINA BORJA , KATARINA PERSSONKATARINA PERSSON , ALISON F. BRADINGALISON F. BRADING , and KARL-ERIK ANDERSSONKARL-ERIK ANDERSSON View All Author Informationhttps://doi.org/10.1097/01.ju.0000055607.67581.09AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: While we have recently detected neuronal nitric oxide synthase (nNOS) immunoreactivity in a heterogeneous population of human male urethral striated muscle, to our knowledge the association of nNOS in the female counterpart is unknown. We investigated the association of nNOS with female urethral striated muscle and re-investigated muscle fiber types. Materials and Methods: Cryostat sections were taken from the middle third of 4 human female urethras. Nicotinamide adenine dinucleotide phosphate diaphorase histochemistry and nNOS immunohistochemistry were performed. Muscle fiber types were identified by myofibrillar adenosine triphosphatase histochemistry and fast twitch troponin T immunohistochemistry. The association between nNOS immunoreactivity and muscle fiber type was analyzed. Results: Positive staining for nicotinamide adenine dinucleotide phosphate diaphorase histochemistry and nNOS immunoreactivity were recognized in the sarcolemma of 43.9% of female urethral striated muscle fibers. Immunoreactivity for fast twitch troponin T immunohistochemistry was demonstrated by 2% of the striated fibers. The use of myofibrillar adenosine triphosphatase showed that all fibers darkly stained uniformly at a pH of 9.6, 4.6 and 4.3, suggesting that they were myofibrillar intermediate muscle fibers. The results allowed the differentiation of 2 subgroups of fibers, namely smaller fibers (modal diameter 10.1 to 15.0 μm.) without nNOS immunoreactivity and larger fibers (modal diameter 15.1 to 20.0 μm.) with nNOS immunoreactivity. Conclusions: To our knowledge female urethral striated muscle has for the first time been found to consist of myofibrillar intermediate fibers and nNOS was positively localized in the sarcolemma of a subgroup of the fibers. This study provides a basis for further investigation into female urethral striated sphincter function and changes in pathological conditions. References 1 : Structural support of the urethra as it relates to stress urinary incontinence: the hammock hypothesis. Am J Obstet Gynecol1994; 170: 1713. 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Google Scholar From the Oxford Continence Group, University Department of Pharmacology and Department of Statistics, University of Oxford, Oxford, United Kingdom, and Department of Clinical Pharmacology, Lund University Hospital, Lund, Sweden© 2003 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited byDeLancey J, Trowbridge E, Miller J, Morgan D, Guire K, Fenner D, Weadock W and Ashton-Miller J (2008) Stress Urinary Incontinence: Relative Importance of Urethral Support and Urethral Closure PressureJournal of Urology, VOL. 179, NO. 6, (2286-2290), Online publication date: 1-Jun-2008.Zini L, Lecoeur C, Swieb S, Combrisson H, Delmas V, Ghérardi R, Abbou C, Chopin D and Yiou R (2018) The Striated Urethral Sphincter of the Pig Shows Morphological and Functional Characteristics Essential for the Evaluation of Treatments for Sphincter InsufficiencyJournal of Urology, VOL. 176, NO. 6, (2729-2735), Online publication date: 1-Dec-2006.ANDRICH D, RICKARDS D, LANDON D, FOWLER C and MUNDY A (2018) STRUCTURAL ASSESSMENT OF THE URETHRAL SPHINCTER IN WOMEN WITH URINARY RETENTIONJournal of Urology, VOL. 173, NO. 4, (1246-1251), Online publication date: 1-Apr-2005. Volume 169Issue 6June 2003Page: 2407-2411 Advertisement Copyright & Permissions© 2003 by American Urological Association, Inc.Keywordsmuscle fibersurethramuscle, skeletalnitric-oxide synthaseMetricsAuthor Information KOSSEN M.T. HO More articles by this author MARIO CORTINA BORJA Financial interest and/or other relationship with University College London. More articles by this author KATARINA PERSSON More articles by this author ALISON F. BRADING Financial interest and/or other relationship with GlaxoSmith Kline, Abbott and Pfizer. More articles by this author KARL-ERIK ANDERSSON More articles by this author Expand All Advertisement PDF downloadLoading ...