Imaging of biological macromolecules on mica in humid air by scanning electrochemical microscopy
1999; National Academy of Sciences; Volume: 96; Issue: 25 Linguagem: Inglês
10.1073/pnas.96.25.14222
ISSN1091-6490
Autores Tópico(s)Analytical Chemistry and Sensors
ResumoImaging of DNA, keyhole limpet hemocyanin, mouse monoclonal IgG, and glucose oxidase on a mica substrate has been accomplished by scanning electrochemical microscopy with a tungsten tip. The technique requires the use of a high relative humidity to form a thin film of water on the mica surface that allows electrochemical reactions to take place at the tip and produce a faradaic current (≈1 pA) that can be used to control tip position. The effect of relative humidity and surface pretreatment with buffer solutions on the ionic conductivity of a mica surface was investigated to find appropriate conditions for imaging. Resolution of the order of 1 nm was obtained.
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