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Robust biological nitrogen fixation in a model grass–bacterial association

2015; Wiley; Volume: 81; Issue: 6 Linguagem: Inglês

10.1111/tpj.12777

1365-313X

Vânia C. S. Pankievicz, Fernanda Plucani do Amaral, Karina Freire D'eça Nogueira Santos, Beverly J. Agtuca, Youwen Xu, Michael J. Schueller, Ana Carolina Maisonnave Arisi, Maria Berenice Reynaud Steffens, Emanuel Maltempi de Souza, Fábio O. Pedrosa, Gary Stacey, Richard A. Ferrieri,

Plant-Microbe Interactions and Immunity

Summary Nitrogen‐fixing rhizobacteria can promote plant growth; however, it is controversial whether biological nitrogen fixation ( BNF ) from associative interaction contributes to growth promotion. The roots of Setaria viridis , a model C 4 grass, were effectively colonized by bacterial inoculants resulting in a significant enhancement of growth. Nitrogen‐13 tracer studies provided direct evidence for tracer uptake by the host plant and incorporation into protein. Indeed, plants showed robust growth under nitrogen‐limiting conditions when inoculated with an ammonium‐excreting strain of Azospirillum brasilense . 11 C‐labeling experiments showed that patterns in central carbon metabolism and resource allocation exhibited by nitrogen‐starved plants were largely reversed by bacterial inoculation, such that they resembled plants grown under nitrogen‐sufficient conditions. Adoption of S. viridis as a model should promote research into the mechanisms of associative nitrogen fixation with the ultimate goal of greater adoption of BNF for sustainable crop production.