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Site‐directed mutagenesis of ricin A chain Trp 211 to Phe

1990; Wiley; Volume: 35; Issue: 4 Linguagem: Inglês

10.1111/j.1399-3011.1990.tb00062.x

0367-8377

Janice L. Bradley, Peter M. McGuire,

Glycosylation and Glycoproteins Research

Oligonucleotide‐directed site‐specific mutagenesis has been used to make a conservative change in the putative active site of the catalytic subunit of the toxin ricin. The substitution of phenylalanine for tryptophan at position 211 of the A chain reduces but does not abolish catalytic activity, as assayed by inhibition of cell‐free protein synthesis.