Portal de Periódicos da CAPES

Identification of Hydrophobic Tags for the Degradation of Stabilized Proteins

2012; Wiley; Volume: 13; Issue: 4 Linguagem: Inglês

10.1002/cbic.201100793

1439-7633

Hyun Seop Tae, Thomas B. Sundberg, Taavi K. Neklesa, Devin J. Noblin, Jeffrey L. Gustafson, Anke G. Roth, Kanak Raina, Craig M. Crews,

Ubiquitin and proteasome pathways

New HyTs are a knockout: We previously reported that labeling HaloTag proteins with low molecular weight hydrophobic tags (HyTs) leads to targeted degradation of HaloTag fusion proteins. In this report, we employed a chemical approach to extend this hydrophobic tagging methodology to highly stabilized proteins by synthesizing and evaluating a library of HyTs, which led to the identification of HyT36. Detailed facts of importance to specialist readers are published as "Supporting Information". Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.