Molecular Crosstalk Between Mechanical and Electrical Junctions at the Intercalated Disc
2007; Lippincott Williams & Wilkins; Volume: 101; Issue: 7 Linguagem: Inglês
10.1161/circresaha.107.161901
ISSN1524-4571
Autores Tópico(s)Ion channel regulation and function
ResumoHomeCirculation ResearchVol. 101, No. 7Molecular Crosstalk Between Mechanical and Electrical Junctions at the Intercalated Disc Free AccessEditorialPDF/EPUBAboutView PDFView EPUBSections ToolsAdd to favoritesDownload citationsTrack citationsPermissions ShareShare onFacebookTwitterLinked InMendeleyReddit Jump toFree AccessEditorialPDF/EPUBMolecular Crosstalk Between Mechanical and Electrical Junctions at the Intercalated Disc Stephan Rohr Stephan RohrStephan Rohr From the Department of Physiology, University of Bern, Switzerland. Originally published28 Sep 2007https://doi.org/10.1161/CIRCRESAHA.107.161901Circulation Research. 2007;101:637–639Since the first electron-microscopic description of the intercalated disc over half a century ago, it has become increasingly clear that this apparently simple boundary between individual cardiomyocytes exhibits a highly complex structural and molecular makeup which conveys a number of different key functions. Adherens junctions and desmosomes ("adhesion junctions") within the intercalated disc are tied up by linker proteins to cytoskeletal proteins of neighboring cardiomyocytes, thus resulting in a network of proteins which is strong enough to absorb mechanical forces exerted during contraction. Secondly, gap junctions consisting of tightly packed connexins permit intercellular exchange of small molecules and excitatory current flow between neighboring cells, the latter being a prerequisite for the safe and rapid propagation of electrical activation along the network of cardiomyocytes. Thirdly, there exists evidence that a number of ion channels like sodium channels and potassium channels are targeted to the intercalated disc with as yet not fully defined functional consequences for the excitation process of cardiac tissue.1 Whereas all of these components of the intercalated disc are topologically segregated and serve distinct functions, recent studies of gene mutations which afflict structural proteins of the intercalated disc suggest that there exists extensive crosstalk between both mechanical and electrical junctions at the molecular level which ultimately can predispose the heart to arrhythmias.The Importance of Adhesion Junctions for Gap Junction Formation and MaintenanceWhereas the topological segregation of adhesion junctions and gap junctions might be interpreted as a sign of structural independence between these 2 major components of the intercalated disc, a number of observations in fact suggests that the presence of stable mechanical contacts based on adhesion junctions is of paramount importance for both the formation and stabilization of functional gap junctional plaques.2 Evidence in support of this hypothesis stems, eg, from the in vitro observation that the establishment of adhesion junctions consisting of N-cadherin, desmoplakin, α-catenin, and plakoglobin precedes the formation of gap junctional plaques between cultured adult rat ventricular cardiomyocytes.3 Once mature gap junctional plaques are formed, it can be intuitively appreciated that they must be further mechanically stabilized because the membrane regions containing gap junctions are relatively rigid and are constantly exposed to shear stress during contractions which might cause disruption of the complexes. The importance of such continued mechanical stabilization is illustrated by the finding that dominant negative suppression of N-cadherin in cultured adult rat cardiomyocytes causes destabilization of adhesion junctions and results in disruption of cell–cell contacts and the disassembly of gap junctions.4 Similarly, induced deletion of the N-cadherin gene in adult mice was shown to lead to a disassembly of the intercalated disc structure with a prominent decrease in levels of connexin43.5 Thus, presently available evidence is in favor of the view that both the formation and maintenance of gap junction plaques is critically dependent on the presence of sufficient mechanical stabilization of the intercalated disc by adhesion junctions. This interdependence between mechanical and electrical junctions seems to be unilateral in the sense that, as shown in an animal model with cardiac specific conditional knockout of connexin43, the absence of connexin43 does not seem to change the structure of the intercalated disc in respect to the spatial organization of adherens junctions and desmosomes.6Gene Mutations, Defects in Intercalated Disc Proteins, and ArrhythmiasApart from basic science considerations, the concept that mechanical stabilization of intercalated discs by protein components of adhesion junctions is a prerequisite for gap junction plaque formation and preservation is gaining increasing support from the analysis of gene mutations involved in a number of hereditary cardiac diseases like dilated cardiomyopathy and arrhythmogenic right ventricular cardiomyopathy (ARVC).7 In a significant number of these cardiac diseases, mutations affect structural proteins of the intercalated disc which are present both in adherens junctions (plakoglobin and vinculin/metavinculin) and desmosomes (plakoglobin, desmoplakin, desmoglein and plakophilin-2). For the case of defects in desmoplakin (Carvajal syndrome) and plakoglobin (Naxos disease), investigations of the ultrastructure of cardiac tissue have revealed substantial abnormalities in intercalated discs and a reduction in the size and abundance of gap junctional plaques.8,9 Ultrastructural abnormalities of the intercalated disc have recently also been described for ARVC related mutations in the desmoglein-2 gene which causes defects in the desmosomal protein desmoglein.10 In all of these cases, precipitation of arrhythmias might be facilitated by the reduction in intercellular gap junctional communication which compromises impulse conduction by inducing slow and possibly discontinuous conduction. For those diseases of the adhesion junctions which additionally exhibit fibrofatty infiltrations, nonuniformities within the electrical substrate at the multicellular level might, analogous to fibrotic hearts, contribute to arrhythmogenesis by inducing discontinuous conduction and conduction blocks.In the present issue of Circulation Research, Oxford and colleagues extend the concept that the integrity of mechanical junctions is important for the proper distribution and function of the gap junction protein connexin43 to yet another protein component of desmosomes which is afflicted by ARVC related gene mutations, ie, plakophilin-2.11Defects in Plakophilin-2 Affect Gap Junctional CommunicationAmong the plakophilins 1 to 3, only plakophilin-2 (PKP2) is present in desmosomes of the intercalated disc. PKP2 belongs to the armadillo family of proteins. It can bind to a large number of desmosomal proteins, including desmoplakin, plakoglobin, the desmogleins, and the desmocollins.12 Moreover, PKP2 was shown to also interact with α-catenin suggesting that it might be part of adherens junctions as well.13 PKP2 gene ablation in mice results in lethal alterations in heart morphogenesis characterized by defects in intercellular adhesion and wall ruptures in mid-gestation.14 Mutations in the PKP2 gene are relatively common in ARVC with incidence rates varying among different studies.15,16 In their in vitro study, Oxford et al now describe structural and functional implications of a reduction of PKP2 on connexin43 expression and gap junction formation in cultured cardiomyocytes and epicardium derived cells.11 They show convincingly, using small interference RNA techniques, that inhibition of PKP2 expression in primary cultures of neonatal rat ventricular cardiomyocytes leads to a reduction of connexin43 content, a redistribution of connexin43 from the intercalated disc to intracellular pools, and a remodeling of desmin and desmoplakin. Functionally, gap junctional remodeling after suppression of PKP2 expression is accompanied by a significant reduction of intercellular dye transfer which indicates compromised intercellular communication. Thus, at least in vitro, these results represent a further piece of evidence in favor of the concept that the integrity of adhesion junctions at the intercalated disc is a prerequisite for the normal targeting and function of connexin43.Interestingly, Oxford and colleagues observe gap junctional remodeling also in the case of PKP2 suppression in epicardium-derived epitheloid cells which, in vitro, are not exposed to the mechanical stress of phasic contractions.11 Intuitively, stabilization of cell–cell contacts in this "static" cells seems less important than for contracting cardiomyocytes. Nevertheless, epicardium-derived cells undergo remodeling of gap junctions after PKP2 suppression which is highly similar to that observed in cardiomyocytes. This suggests that either (1) static tensions exerted by the cytoskeletons of neighboring epicardium-derived cells is still sufficient as to cause reorganization of the cell–cell contacts in the absence of PKP2, or that (2) PKP2 is directly involved in the stabilization of connexins within the gap junctional plaque. The latter possibility is supported by pulldown experiments showing that PKP2 associates with connexin43. In this respect, PKP2 represents of further member of the list of binding partners of connexin43 (zonula occludens-1, v-Src, c-Src, α-catenin, β-catenin, caveolin-1, tubulin)17 which are thought to be involved in connexin43 trafficking, stabilization, and shaping of the junctional plaque.18Given that PKP2 is both a component of the desmosomes and seems to be capable of direct molecular interaction with connexin43, it will be of future interest to sort out the differential involvement of this protein (1) in connexin43 trafficking, (2) in the direct stabilization of gap junctional plaques, and (3) in the mechanical stabilization of the intercalated disc by adhesion junctions. This knowledge will help to advance our understanding of the complex influence that molecular constituents of mechanical junctions exert on the formation and preservation of gap junctional plaques.The opinions expressed in this editorial are not necessarily those of the editors or of the American Heart Association.I wish to express my gratitude to André G. Kléber for helpful discussions on this manuscript.Sources of FundingThis work was funded by the Swiss National Science Foundation (grant# 3100AO-105916 to S.R.) and the "Heart Remodeling in Health and Disease" project of the Swiss University Conference.DisclosuresNone.FootnotesCorrespondence to Stephan Rohr, MD, Department of Physiology, University of Bern, Bühlplatz 5, CH-3012 Bern, Switzerland. E-mail [email protected] References 1 Kucera JP, Rohr S, Rudy Y. Localization of sodium channels in intercalated disks modulates cardiac conduction. Circ Res. 2002; 91: 1176–1182.LinkGoogle Scholar2 Saffitz JE. Adhesion molecules: Why they are important to the electrophysiologist. J Cardiovasc Electrophys. 2006; 17: 225–229.CrossrefMedlineGoogle Scholar3 Kostin S, Hein S, Bauer EP, Schaper J. Spatiotemporal development and distribution of intercellular junctions in adult rat cardiomyocytes in culture. Circ Res. 1999; 85: 154–167.CrossrefMedlineGoogle Scholar4 Hertig CM, Eppenberger-Eberhardt M, Koch S, Eppenberger HM. N-cadherin in adult rat cardiomyocytes in culture. I. Functional role of N-cadherin and impairment of cell-cell contact by a truncated N-cadherin mutant. J Cell Sci. 1996; 109: 1–10.CrossrefMedlineGoogle Scholar5 Kostetskii I, Li J, Xiong Y, Zhou R, Ferrari VA, Patel VV, Molkentin JD, Radice GL. Induced deletion of the N-cadherin gene in the heart leads to dissolution of the intercalated disc structure. Circ Res. 2005; 96: 346–354.LinkGoogle Scholar6 Gutstein DE, Liu F-Y, Meyers MB, Choo A, Fishman GI. The organization of adherens junctions and desmosomes at the cardiac intercalated disc is independent of gap junctions. J Cell Sci. 2003; 116: 875–885.CrossrefMedlineGoogle Scholar7 Li J, Patel VV, Radice GL. Dysregulation of cell adhesion proteins and cardiac arrhythmogenesis. Clin Med and Res. 2006; 4: 42–52.CrossrefMedlineGoogle Scholar8 Kaplan SR, Gard JJ, Carvajal-Huerta L, Ruiz-Cabezas JC, Thiene G, Saffitz JE. Structural and molecular pathology of the heart in Carvajal syndrome. Cardiovasc Pathol. 2004; 13: 26–32.CrossrefMedlineGoogle Scholar9 Kaplan SR, Gard JJ, Protonotarios N, Tsatsopoulou A, Spiliopoulou C, Anastasakis A, Squarcioni CP, McKenna WJ, Thiene G, Basso C, Brousse N, Fontaine G, Saffitz JE. Remodeling of myocyte gap junctions in arrhythmogenic right ventricular cardiomyopathy due to a deletion in plakoglobin (Naxos disease). 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A unique and specific interaction between αT-catenin and plakophilin-2 in the area composita, the mixed-type junctional structure of cardiac intercalated discs. J Cell Sci. 2007; 120: 2126–2136.CrossrefMedlineGoogle Scholar14 Grossmann KS, Grund C, Huelsken J, Behrend M, Erdmann B, Franke WW, Birchmeier W. Requirement of plakophilin 2 for heart morphogenesis and cardiac junction formation. J Cell Biol. 2004; 167: 149–160.CrossrefMedlineGoogle Scholar15 Sen-Chowdhry S, Syrris P, Ward D, Asimaki A, Sevdalis E, McKenna WJ. Clinical and genetic characterization of families with arrhythmogenic right ventricular dysplasia/cardiomyopathy provides novel insights into patterns of disease expression. Circulation. 2007; 115: 1710–1720.LinkGoogle Scholar16 van Tintelen JP, Entius MM, Bhuiyan ZA, Jongbloed R, Wiesfeld ACP, Wilde AAM, van der Smagt J, Boven LG, Mannens MMAM, van Langen IM, Hofstra RMW, Otterspoor LC, Doevendans PAFM, Rodriguez L-M, van Gelder IC, Hauer RNW. 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Severs N, Bruce A, Dupont E and Rothery S (2008) Remodelling of gap junctions and connexin expression in diseased myocardium, Cardiovascular Research, 10.1093/cvr/cvn133, 80:1, (9-19) Boogerd C, Lacraz G, Vértesy Á, van Kampen S, Perini I, de Ruiter H, Versteeg D, Brodehl A, van der Kraak P, Giacca M, de Jonge N, Junker J, van Oudenaarden A, Vink A and van Rooij E (2022) Spatial transcriptomics unveils ZBTB11 as a regulator of cardiomyocyte degeneration in arrhythmogenic cardiomyopathy, Cardiovascular Research, 10.1093/cvr/cvac072 September 28, 2007Vol 101, Issue 7 Advertisement Article InformationMetrics https://doi.org/10.1161/CIRCRESAHA.107.161901PMID: 17901364 Originally publishedSeptember 28, 2007 PDF download Advertisement
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