Portal de Periódicos da CAPES

Localization of CD4 and CCR5 in Living Cells

2003; American Society for Microbiology; Volume: 77; Issue: 8 Linguagem: Inglês

10.1128/jvi.77.8.4985-4991.2003

1098-5514

Carolyn M. Steffens, Thomas J. Hope,

RNA Interference and Gene Delivery

ABSTRACT The events preceding human immunodeficiency virus fusion and entry are influenced by the concentration and distribution of receptor and coreceptor molecules on the cell surface. However, the extent to which these proteins colocalize with one another in the cell membrane remains unclear. Using high-resolution deconvolution fluorescent microscopy of living cells, we found that both CD4 and CCR5 accumulate in protruding membrane structures containing actin and ezrin. Although CD4 and CCR5 extensively colocalize in these structures, they do not exist in a stable complex.