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Vitrification of human 8-cell embryos, a modified protocol for better pregnancy rates

2005; Elsevier BV; Volume: 11; Issue: 4 Linguagem: Inglês

10.1016/s1472-6483(10)61135-2

1472-6491

Gottumukkala Achyuta Rama Raju, G. B. Haranath, K. Murali Krishna, G. Jaya Prakash, K. Madan,

Ovarian function and disorders

Human day 3 embryos were vitrified in modified ethylene glycol-based protocol. The present study reports a post-thaw survival rate of 95.3%, which was significantly higher than that of the conventional slow-freezing protocol (60.0%, P < 0.05). The implantation and pregnancy rates were also higher (14.9% and 35.0%) than that in the slow-freezing protocol (4.2% and 17.4%). On average, three embryos were transferred per patient. Out of the 40 transfer cycles, five deliveries and nine ongoing pregnancies were achieved. Four ended with delivery of a singleton and one a miscarriage. Ethylene glycol is a good cryoprotectant to preserve human 8-cell embryos because of its low toxicity as shown by the high survival rate, and vitrification is a promising alternate to the conventional slow-freezing method.