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THE FORMATION OF PHOSPHOLIPIDS FROM C 14 -LABELLED PRECURSORS IN SLICES FROM IMMATURE RAT BRAIN

1962; Canadian Science Publishing; Volume: 40; Issue: 3 Linguagem: Inglês

10.1139/o62-039

0576-5544

E.T. Pritchard,

Metabolomics and Mass Spectrometry Studies

The relative incorporation of several C 14 -labelled compounds into the phospholipid and free cholesterol of developing rat brain slices at various ages from 1 to 19 days was examined. Experiments have shown that labelling of saturated fatty acids of phospholipids and cholesterol from acetate-1-C 14 and propanoate-1-C 14 was much more in 1- to 9-day animals than in older animals while the labelling from non-fatty acid precursors such as ethanolamine-1,2-C 14 and choline-Me-C 14 was not greatly affected by the aging processes. Palmitic acid was the major acid labelled by acetate, but both stearic and myristic acids received appreciable activity.The results tend to indicate that the saturated fatty acids of rat brain phospholipids are formed rapidly at a very early stage in brain development. The activity of this synthetic system then appears to decrease to the low level apparent in the adult rat. The glycerol–phosphate-base moieties of the phospholipids, on the other hand, appear to retain their relatively high replacement activity at all ages.