Monocyte chemotactic protein-1 (MCP-1) acts as a paracrine and autocrine factor for prostate cancer growth and invasion
2006; Wiley; Volume: 66; Issue: 12 Linguagem: Inglês
10.1002/pros.20464
ISSN1097-0045
AutoresYi Lu, Zhong Cai, Deborah L. Galson, Guozhi Xiao, Yulin Liu, Diane George, Mona F. Melhem, Zhi Yao, Jian Zhang,
Tópico(s)Cell Adhesion Molecules Research
ResumoMonocyte chemotactic protein-1 (MCP-1) plays a key role in the recruitment and activation of monocytes during inflammation. Increased MCP-1 serum levels in patients with various cancers were correlated with advanced stage. Here, we evaluated the role of MCP-1 on prostate cancer (CaP) cell proliferation and invasion.Expression of MCP-1 in tissue specimens was analyzed by immunohistochemical staining. MCP-1 production was determined by ELISA in conditioned media collected from primary prostate epithelia (PrEC), LNCaP, C4-2B, PC3 cells, and hFOB. Cell proliferation and invasion were assayed by MTS assay and invasion chambers.All CaP cells, as well as hFOB, produced high amount of MCP-1 compared to PrEC cells. MCP-1 expression levels were associated with advanced pathologic stage. MCP-1 induced proliferation and invasion of CaP cells and this was abolished partially either by CCR2 antagonist or PI3 Kinase inhibitor.MCP-1 acts as a paracrine and autocrine factor for CaP growth and invasion.
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