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Methanofollis ethanolicus sp. nov., an ethanol-utilizing methanogen isolated from a lotus field

2009; Microbiology Society; Volume: 59; Issue: 4 Linguagem: Inglês

10.1099/ijs.0.003731-0

1466-5034

Hiroyuki Imachi, Sanae Sakai, Hiroki Nagai, Takashi Yamaguchi, Ken Takai,

Genomics and Phylogenetic Studies

A novel methane-producing archaeon, designated strain HASUT, was isolated from a lotus field. Cells were Gram-negative, non-motile, irregular cocci, 2–3 μm in diameter, and occurred singly. Growth was observed at 15–40 °C (optimum, 37 °C) and pH 6.5–7.5 (optimum, pH 7.0). The G+C content of the genomic DNA was 60.9 mol%. Strain HASUT utilized ethanol, 1-propanol, 1-butanol, hydrogen and formate for growth and methane production. It converted ethanol to methane and acetate. Based on comparative 16S rRNA gene sequence analysis, strain HASUT was shown to be affiliated with the genus Methanofollis. It was related most closely to the type strain of Methanofollis liminatans (96.1 % 16S rRNA gene sequence similarity). Based on phylogenetic analysis and phenotypic characteristics, strain HASUT is considered to represent a novel species of the genus Methanofollis, for which the name Methanofollis ethanolicus sp. nov. is proposed. The type strain is HASUT (=NBRC 104120T=JCM 15103T=DSM 21041T).