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The Effect of Agitation upon the Livability of Bovine Spermatozoa

1948; Elsevier BV; Volume: 31; Issue: 10 Linguagem: Inglês

10.3168/jds.s0022-0302(48)92268-1

1529-9066

P.W. Prince, J.O. Almquist,

Sperm and Testicular Function

In the routine operation of most artificial breeding associations, diluted semen samples are transported from the central bull stud to the outlying inseminators, and often considerable distances are involved.Various means of transportation are employed and it is obvious that the semen samples are subjected to different amounts of agitation enroute.Bretschneider (2) reported that the vigorous shaking of bull semen for 3 minutes destroyed motility.Spermatozoa from the normal ejaculate showed more resistance to shaking than did those secured from the testicle or epididymis.Smirnov-Ugrjumov n4) observed that the transportation of undiluted bull semen in thermos flasks at 15-20°O.for distances of 0.6 to 5.9 miles brought about a reduction in spermatozoan activity.Hronopulo (6) reported that transportation of undiluted bull semen did not affect the fertility of the semen during the first 4 hours of storage.After a storage period of 4 hours, however, the fertility of semen transported distances greater than 21.7 miles was markedly reduced.Ayyar (1) noted that hand shaking during transport killed bull spermatozoa.Several workers have noted effects of agitation in connection with studies of semen physiology.Gunn (4) reported that periodic shaking of ram semen contained in rubber-stoppered test tubes was effective in providing the aeration which he considered necessary for maintenance of spermatozoan motility.Motility was maintained even when shaking was vigorous.On the other hand, Nagornyi and Smirnov (11) found that the resistance of ram spermatozoa to sodium chloride was decreased by continuous agitation.During the course of metabolism studies with ram and bull semen, Mann (10) observed that when a suspension of spermatozoa was shaken vigorously in the presence of air, the cytochrome enzyme within the cell was oxidized rapidly.In the process of examining samples of diluted semen shipped to this laboratory from the several artificial breeding cooperatives in Pennsylvania, it was