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Acute and topic anti-edematogenic fractions isolated from the seeds of Pterodon pubescens

2004; Oxford University Press; Volume: 56; Issue: 1 Linguagem: Inglês

10.1211/0022357022485

2042-7158

M C C Silva, Carlos Roberto Machado Gayer, Cláudia S. Lopes, N O Calixto, Patrícia A. Reis, Caroline Passaes, Márcia Cristina Paes, Sergio R. Dalmau, K.C.C. Sabino, Adriane R. Todeschini, Marsen Garcia Pinto Coelho,

Bioactive Natural Diterpenoids Research

Abstract We previously demonstrated that alcoholic extracts from Pterodon pubescens Benth. (Sucupira branca, Leguminosae) seeds exhibit anti-arthritic activity. In the present work we show that the oleaginous extract obtained from P. pubescens seeds (OEP) exhibits acute or topic anti-edematogenic activity when tested in carrageenan-induced paw edema or in croton oil-induced ear edema assays, respectively. Four fractions were obtained from OEP by sequential liquid–liquid extraction. The anti-edematogenic properties were predominant in the hexanic fraction, which was further fractionated by HPLC, yielding three sub-fractions (PF1.1, PF1.2 and PF1.3). PF1.1 and PF1.3 showed potent acute and topic anti-edematogenic activity. The PF1.2 sub-fraction, although not active in the carrageenan assay, exhibited a potent anti-edematogenic activity in the croton oil-induced ear edema. This sub-fraction shows a maximum efficacy similar to indometacin in a lower dose. The PF1.1 sub-fraction presented a complex mixture containing furane diterpene derivatives of vouacapan. PF1.2 consists of a single substance, geranylgeraniol, as determined by GC/MS and NMR, while PF1.3 contains farnesol.