A green fluorescent chemosensor for amino acids provides a versatile high-throughput screening (HTS) assay for proteases
2003; Elsevier BV; Volume: 13; Issue: 10 Linguagem: Inglês
10.1016/s0960-894x(03)00280-4
ISSN1464-3405
AutoresKathryn E. S. Dean, Gérard Klein, Olivier Renaudet, Jean‐Louis Reymond,
Tópico(s)Electrochemical sensors and biosensors
ResumoThe water soluble fluorescein-based ligand 1 forms a non-fluorescent complex with Cu2+. This complex serves as a fluorescent sensor for amino acids in the 10−3 M concentration range. Since the signal response is very fast, the sensor can be used to detect the hydrolytic activity of various proteases (trypsin, chymotrypsin, subtilisin) on bovine serum albumin as a whole protein substrate, and more generally to follow reactions releasing or removing free amino acids, in real time.
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