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Folding and stability of integral membrane proteins in amphipols

2014; Elsevier BV; Volume: 564; Linguagem: Inglês

10.1016/j.abb.2014.10.013

1096-0384

Jörg H. Kleinschmidt, Jean‐Luc Popot,

RNA and protein synthesis mechanisms

Amphipols (APols) are a family of amphipathic polymers designed to keep transmembrane proteins (TMPs) soluble in aqueous solutions in the absence of detergent. APols have proven remarkably efficient at (i) stabilizing TMPs, as compared to detergent solutions, and (ii) folding them from a denatured state to a native, functional one. The underlying physical–chemical mechanisms are discussed.