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Identification of upregulated genes in oral squamous cell carcinomas

2012; Wiley; Volume: 35; Issue: 10 Linguagem: Inglês

10.1002/hed.23169

1097-0347

Roberta Cardim Lessa, Antônio Hugo José Fróes Marques Campos, Carlos Elias de Freitas, Felipe Rodrigues da Silva, Luiz Paulo Kowalski, André Lopes Carvalho, André L. Vettore,

Molecular Biology Techniques and Applications

Background Oral cancer is the most common subset of head and neck squamous cell carcinomas (HNSCC). These tumors often have an aggressive clinical outcome hallmarked by a propensity for local invasion and regional nodal metastasis. Upregulated genes could be useful as markers for diagnosis, prognosis, and as new drug targets for these tumors. Methods To identify upregulated genes in oral squamous cell carcinomas (OSSCs), we examined the ORESTES public database and used a quantitative reverse transcription–polymerase chain reaction (qRT‐PCR) approach to determine the expression level of selected genes in tumor samples. Results and Conclusions The ORESTES data mining analysis indicated 40 upregulated genes in HNSCC. Nine of these candidate genes were selected for further qRT‐PCR validation and 3 of them ( ALDOA , AHSA1 , and POLQ ) were frequently found upregulated in OSCC samples, which may indicate an association of these genes with the carcinogenesis process in this tumor site and they can constitute potential new targets for therapy. © 2012 Wiley Periodicals, Inc. Head Neck 35: 1475–1481, 2013