Evaluation of homogeneous high-density lipoprotein cholesterol assay on a BM/Hitachi 747-200 analyzer.
1998; National Institutes of Health; Volume: 44; Issue: 5 Linguagem: Inglês
Autores
Maun-Jan Lin, Carolyn Hoke, Bruce Ettinger,
Tópico(s)Lipid metabolism and disorders
ResumoBecause of the inverse correlation that exists between serum HDL-C concentration and risk of atherosclerotic disease (1), monitoring of high-density lipoprotein cholesterol (HDL-C) in serum is clinically important. In 1993, the National Cholesterol Education Program Adult Treatment Panel II (NCEP ATP II) revised its guidelines for diagnosis and treatment of hypercholesterolemia in adults to include HDL-C measurement at the initial screening stage when total cholesterol is measured (2). The enhanced role of HDL-C in clinical practice increases the need for reliable and readily performable HDL-C measurement. Most routine laboratories use a two-step method: chemical precipitation of lipoproteins containing apoprotein B, then quantification of HDL as cholesterol remaining in the supernate. Such precipitation-based methods are time-consuming, labor-intensive, require relatively large volumes of serum, and cannot be fully automated. Recently, several methods of direct HDL-C measurement have become commercially available, including the use of magnetically responsive particles with polyanion-metal combinations (3), the use of polyethylene glycol (PEG) with antibodies against apoprotein B and apoprotein CIII (4)(5), and the use of PEG-modified enzymes and sulfated α-cyclodextrin (6)(7). Okazaki et al. (8) also recently proposed the use of high-performance liquid chromatography (HPLC) as a tool to compare different methods for HDL-C. The goal of this study was to evaluate three assays for homogeneous HDL-C on BM/Hitachi 747–200 Automatic Analyzer (Boehringer Mannheim Corp.): the direct HDL-cholesterol reagent kit (Boehringer Mannheim Corp.), the N-geneousTM HDL cholesterol reagent kit (Genzyme Diagnostics), and EZ-HDLTM cholesterol reagent kit (Sigma Diagnostics). For each HDL-C assay, the reagent kit contains Reagent 1 and Reagent 2; Reagent 1 is liquid, and Reagent 2 is lyophilized. Reaction principles of these three methods are quite different; the direct-HDL uses PEG-modified cholesterol esterase and cholesterol oxidase as well as sulfated α-cyclodextrin to provide selective determination of HDL-C in serum; …
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