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Putative virulence‐related genes in Vibrio anguillarum identified by random genome sequencing

2006; Wiley; Volume: 29; Issue: 3 Linguagem: Inglês

10.1111/j.1365-2761.2006.00692.x

1365-2761

Channarong Rodkhum, Ikuo Hırono, Michiel Stork, Manuela Di Lorenzo, Jorge H. Crosa, Takashi Aoki,

Identification and Quantification in Food

Abstract The genome of Vibrio anguillarum strain H775‐3 was partially determined by a random sequencing procedure. A total of 2300 clones, 2100 from a plasmid library and 200 from a cosmid library, were sequenced and subjected to homology search by the BLAST algorithm. The total length of the sequenced clones is 1.5 Mbp. The nucleotide sequences were classified into 17 broad functional categories. Forty putative virulence‐related genes were identified, 36 of which are novel in V. anguillarum , including a repeat in toxin gene cluster, haemolysin genes, enterobactin gene, protease genes, lipopolysaccharide biosynthesis genes, capsule biosynthesis gene, flagellar genes and pilus genes.