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Effect of North American Ginseng on 137 Cs‐induced Micronuclei in Human Lymphocytes: A Comparison with WR‐1065

2008; Wiley; Volume: 22; Issue: 12 Linguagem: Inglês

10.1002/ptr.2533

1099-1573

Tung‐Kwang Lee, Weidong Wang, Kevin O’Brien, Roberta M. Johnke, Tao Wang, Ron R. Allison, Angelica L. Diaz,

DNA Repair Mechanisms

Abstract To explore the radioprotective effect of a standardized North American ginseng extract (NAGE) on human peripheral blood lymphocytes (PBL), a micronuclei (MN) assay was conducted in PBL obtained from 12 volunteers. NAGE (50–1000 µg/mL) and WR‐1065 (1 m m and 3 m m ) were applied to PBL cultures at 0 h and 90 min post‐irradiation. It was found that (1) the baseline MN yield of PBL ranged from 14.4 ± 1.5 to 15.9 ± 1.5 per 1000 binucleated cells ( p > 0.05); after irradiation (1 Gy and 2 Gy), the MN yield increased sharply; (2) MN yields declined with increasing concentrations of NAGE and WR‐1065. Even at 90 min post‐irradiation of 1 Gy, the maximum level of MN reduction rate caused by NAGE and WR‐1065 was 53.8% and 59.2%, respectively; after 2 Gy irradiation, it was 37.3% and 42%, respectively; (3) the MN distribution in PBL followed a non‐Poisson distribution in all cases; and (4) both NAGE and WR‐1065 showed no significant effect on the proliferation index of lymphocytes. The results indicate that NAGE is a relatively non‐toxic natural product, which can be administered as a dietary supplement and has the potential to be a radiation countermeasure. Copyright © 2008 John Wiley & Sons, Ltd.