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Trapping of Proteins under Physiological Conditions in a Nanopipette

2005; Wiley; Volume: 44; Issue: 24 Linguagem: Inglês

10.1002/anie.200500196

1521-3773

Richard W. Clarke, Samuel S. White, Dejian Zhou, Liming Ying, David Klenerman,

Microfluidic and Capillary Electrophoresis Applications

Angewandte Chemie International EditionVolume 44, Issue 24 p. 3747-3750 Communication Trapping of Proteins under Physiological Conditions in a Nanopipette† Richard W. Clarke, Richard W. Clarke Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, UK, Fax: (+44) 1223-336-362 These authors contributed equally to this work.Search for more papers by this authorSamuel S. White, Samuel S. White Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, UK, Fax: (+44) 1223-336-362 These authors contributed equally to this work.Search for more papers by this authorDejian Zhou Dr., Dejian Zhou Dr. Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, UK, Fax: (+44) 1223-336-362Search for more papers by this authorLiming Ying Dr., Liming Ying Dr. [email protected] Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, UK, Fax: (+44) 1223-336-362Search for more papers by this authorDavid Klenerman Dr., David Klenerman Dr. [email protected] Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, UK, Fax: (+44) 1223-336-362Search for more papers by this author Richard W. Clarke, Richard W. Clarke Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, UK, Fax: (+44) 1223-336-362 These authors contributed equally to this work.Search for more papers by this authorSamuel S. White, Samuel S. White Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, UK, Fax: (+44) 1223-336-362 These authors contributed equally to this work.Search for more papers by this authorDejian Zhou Dr., Dejian Zhou Dr. Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, UK, Fax: (+44) 1223-336-362Search for more papers by this authorLiming Ying Dr., Liming Ying Dr. [email protected] Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, UK, Fax: (+44) 1223-336-362Search for more papers by this authorDavid Klenerman Dr., David Klenerman Dr. [email protected] Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, UK, Fax: (+44) 1223-336-362Search for more papers by this author First published: 06 June 2005 https://doi.org/10.1002/anie.200500196Citations: 101 † This work was funded by the Biotechnology and Biological Sciences Research Council (BBSRC), UK. We thank Universal Imaging Corporation, UK, for the loan of the Photometrics CCD camera and MetaMorph software. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Graphical Abstract Caught in a trap and on camera: A nanopipette is used for electrodeless dielectrophoresis and clear evidence is shown, by using wide-field fluorescence imaging (see image), for the reversible trapping of Alexa-488-labeled proteins (protein G and immunoglobulin G) and also of the fluorophore alone. 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