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Genome Assembly and Particle Maturation of the Birnavirus Infectious Pancreatic Necrosis Virus

2004; American Society for Microbiology; Volume: 78; Issue: 24 Linguagem: Inglês

10.1128/jvi.78.24.13829-13838.2004

1098-5514

Rodrigo A. Villanueva, José Luis Galaz, Juan Antonio Valdés, M Jashés, Ana María Sandino,

Animal Virus Infections Studies

In this study, we have analyzed the morphogenesis of the birnavirus infectious pancreatic necrosis virus throughout the infective cycle in CHSE-214 cells by using a native agarose electrophoresis system. Two types of viral particles (designated A and B) were identified, isolated, and characterized both molecularly and biologically. Together, our results are consistent with a model of morphogenesis in which the genomic double-stranded RNA is immediately assembled, after synthesis, into a large (66-nm diameter) and uninfectious particle A, where the capsid is composed of both mature and immature viral polypeptides. Upon maturation, particles A yield particles B through the proteolytic cleavage of most of the remaining viral precursors within the capsid, the compaction of the particle (60-nm diameter), and the acquisition of infectivity. These studies will provide the foundation for further analyses of birnavirus particle assembly and RNA replication.