Tag-Mass: Specific Molecular Imaging of Transcriptome and Proteome by Mass Spectrometry Based on Photocleavable Tag
2007; American Chemical Society; Volume: 6; Issue: 6 Linguagem: Inglês
10.1021/pr0700044
ISSN1535-3907
AutoresR. Lemaire, Jonathan Stauber, Maxence Wisztorski, Christelle Van Camp, Annie Desmons, Mathieu C. Deschamps, G. Proess, I. Rudlof, Amina S. Woods, Robert Day, Michel Salzet, Isabelle Fournier,
Tópico(s)Analytical Chemistry and Chromatography
ResumoMALDI tissue imaging of tissues has become a promising technique for tracking biomarkers while determining their location and structural characterization. We have now developed specific targeting probes (oligonucleotides, antibodies), named Tag-Mass. This approach is based on probes modified with a photocleavable linker coupled with a tag cleaved and detected using mass spectrometry. Tag-Mass development is the key for a rapid, sensitive, and accurate approach to correlate levels of expression of different mRNA or proteins in diseases. Keywords: MALDI • specific tissue imaging • Tag-Mass • photocleavage • multiplex • transcriptome • proteome
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